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Chapter 11,035

N-linked oligosaccharide chains of Sendai virus fusion protein determine the interaction with endoplasmic reticulum molecular chaperones

Tamura, T.; Yamashita, T.; Segawa, H.; Taira, H.

FEBS Letters 513(2-3): 153-158

2002


ISSN/ISBN: 0014-5793
PMID: 11904141
DOI: 10.1016/s0014-5793(02)02229-9
Accession: 011034906

The selectivity and individual roles of the N-linked oligosaccharide chains of Sendai virus fusion protein (F protein) in the interaction with endoplasmic reticulum molecular chaperones were investigated by analyses of transient expression of single N-glycosylation mutants and sequential immunoprecipitation. We demonstrated differential interactions depending on the location of the N-linked oligosaccharide chain, and showed that these interactions were correlated with the folding and transport of F proteins. Moreover, mutant F proteins that lacked the specific N-linked oligosaccharide chains required for disulfide bond formation showed increased association with ERp57.

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