+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Neuropilin-2 is a novel marker expressed in pancreatic islet cells and endocrine pancreatic tumours



Neuropilin-2 is a novel marker expressed in pancreatic islet cells and endocrine pancreatic tumours



Journal of Pathology 198(1): 77-82



Neuropilin-2 (NP-2) is a cell surface transmembrane protein originally characterized as a receptor for the type 3 semaphorins, and more recently for a number of vascular endothelial growth factor (VEGF) isoforms. NP-2 expression has been recently localized to a subset of neuroendocrine cells in the gastrointestinal tract. The aim of this study was to define the expression pattern of NP-2 in normal pancreatic islets and to determine the utility of NP-2 expression as a diagnostic marker of pancreatic endocrine tumours. Paraffin-embedded tissue sections from 30 endocrine pancreatic tumours (EPTs) and from normal pancreas were immunostained with a rabbit polyclonal antibody generated towards NP-2. Nineteen of the tumours were hormonally functional (nine insulinomas, nine gastrinomas, and one glucagonoma). The NP-2 staining pattern was correlated with islet cell hormone expression. In addition, NP-2 expression was evaluated in other normal neuroendocrine tissues and neuroendocrine neoplasms. In normal pancreas, NP-2 stained a distinct subset of islet cells situated primarily at the islet periphery. Double immunohistochemical staining revealed co-localization with glucagon-expressing cells. Moderate to strong NP-2 staining was present in 27 of 30 EPTs. Serial staining of the pancreatic tumours with insulin, gastrin, glucagon, pancreatic polypeptide (PP) or somatostatin did not reveal a distinct pattern of co-localization. NP-2 expression was not detected in neuroendocrine cells outside the gastroenteropancreatic system, or in their corresponding neoplasms, except for focal staining in one bronchial carcinoid tumour. In conclusion, the vast majority of EPTs examined expressed NP-2, suggesting its utility as a diagnostic marker for these tumours. The function of NP-2 in islet cell biology or tumourigenesis remains to be elucidated.

(PDF emailed within 0-6 h: $19.90)

Accession: 011049333

Download citation: RISBibTeXText

PMID: 12210066

DOI: 10.1002/path.1179


Related references

Neuropilin-2 is a novel neuroendocrine marker expressed in pancreatic islet cells and islet cell tumors. Laboratory Investigation 81(1): 202A, 2001

Pancreatic polypeptide is a useful plasma marker for radiographically evident pancreatic islet cell tumors in patients with multiple endocrine neoplasia type 1. Surgery 122(6): 1012-9; Discussion 1019-20, 1998

Pancreatic polypeptide is a useful plasma marker for radiographically evident pancreatic islet cell tumors in patients with multiple endocrine neoplasia type 1. Surgery (st Louis). 122(6): 1012-1020,., 1997

Oct4 is expressed in Nestin-positive cells as a marker for pancreatic endocrine progenitor. Histochemistry and Cell Biology 131(5): 553-563, 2009

Clusterin is highly expressed in pancreatic endocrine tumours but not in solid pseudopapillary tumours. Histopathology 50(3): 331-337, 2007

The increase in pancreatic endocrine mass after brief occlusion of the main pancreatic duct is primarily due to islet expansion and does not solely originate from islet neogenesis. Pancreas 30(1): E1-E9, 2005

Cloning and expression analysis of the porcine pancreatic islet genes in long-term culture using a monolayer cultured pancreatic endocrine cells. Diabetes 50(Supplement 2): A340, 2001

Pancreatic islet and stellate cells are the main sources of endocrine gland-derived vascular endothelial growth factor/prokineticin-1 in pancreatic cancer. Pancreatology 9(1-2): 165-172, 2009

Two distinct types of islet abnormalities associated with endocrine pancreatic tumours. Virchows Archiv. A, Pathological Anatomy and Histology 376(3): 209-219, 1977

Islet 1 (Isl1) is an excellent marker for pancreatic endocrine tumors (PET) and their metastases. 2007

Progranulin Stimulates Proliferation of Mouse Pancreatic Islet Cells and Is Overexpressed in the Endocrine Pancreatic Tissue of an MEN1 Mouse Model. Pancreas 45(4): 533-540, 2016

Possible mechanism of pancreatic b-cell dysfunction by oxidative stress in islet graft: The role of vascular cell adhesion molecule-1 (VCAM-1) expressed on pancreatic b-cells. 2007

Islet 1 (Isl1) expression is a reliable marker for pancreatic endocrine tumors and their metastases. American Journal of Surgical Pathology 32(3): 420-425, 2008

Telomerase activity in pancreatic endocrine tumours: a potential marker for malignancy. Molecular Pathology 53(3): 133-136, 2000

Characterization of a novel cell surface glyco protein co expressed on cells rearing the insulin receptor and selectively expressed on pancreatic islet cells. Clinical Research: 391a, 1982