+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Non-invasive collection of ejaculates from the common marmoset (Callithrix jacchus) using penile vibrostimulation



Non-invasive collection of ejaculates from the common marmoset (Callithrix jacchus) using penile vibrostimulation



American Journal of Primatology 52(3): 149-154



Penile vibrostimulation (PVS), a noninvasive repeatable method, has been shown in the squirrel monkey to yield semen of higher quality than rectal probe electro-ejaculation (RPE). The present study aimed at establishing the conditions for PVS to collect ejaculates from marmoset monkeys. Ten adult males were trained on the appropriate handling before each was subject to six to 12 PVS tests. Ejaculation was stimulated using a FertiCare personal vibrator fitted with a 2 cm x 0.5 cm i.d. glass tube. The stimulus was repeatedly applied over a frequency of 75-95 Hz and amplitude of 1-2 mm for up to 20 min. Ejaculates were analyzed for volume, total sperm number, sperm concentration, and proportion of living and motile sperm. Ejaculates were obtained in 31 of 88 PVS tests; 87.1% of the ejaculations occurred at 80-85 Hz frequency and 1-1.5 mm amplitude. In 18 tests ejaculates were produced within 49.7 seconds. Ejaculates were characterized by (mean values): volume 31.9 microl, total sperm number 34.2 x 10(6)/ejaculate, concentration 1,154.2 x 10(6) sperm/ml, live sperm 74.6%, motile sperm 59.6%. Total number and concentration of spermatozoa were significantly enhanced in singly living males. PVS yielded three to four times more spermatozoa than comparable previously published values for RPE. Enhancing the success rate by preselecting males for responsiveness may render PVS the sperm collection method of choice in marmoset monkeys.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011065046

Download citation: RISBibTeXText

PMID: 11078029

DOI: 10.1002/1098-2345(200011)52:3<149::aid-ajp4>3.0.co;2-b


Related references

Minimally invasive transabdominal collection of preimplantation embryos from the common marmoset monkey (Callithrix jacchus). Theriogenology 78(4): 811-816, 2012

A non-invasive method of weighing amongst a group of semi free common marmoset Callithrix jacchus Eine nichtinvasive Methode der Gewichtserfassung an einer Gruppe semifreilebender Weissbueschelaffen Callithrix jacchus. Zoologische Garten. Dezember; 736: 422-425, 2003

Ejaculates from the common marmoset (Callithrix jacchus) contain semenogelin and beta-microseminoprotein but not prostate-specific antigen. Molecular Reproduction and Development 71(2): 247-255, 2005

Collection and analysis of semen from the common marmoset (Callithrix jacchus). Journal of Andrology 12(3): 214-220, 1991

Protozoan and helminth parasitism in common marmoset Callithrix jacchus Parasitismo por helmintos e protozoarios no sagui comum Callithrix jacchus. Unknown, 1997

Diurnal variation in common marmoset Callithrix jacchus sociosexual behaviors Variacao diurna em comportamentos socio-sexuais do sagui, Callithrix jacchus. Unknown, 1997

Systems for collection of urine in the captive common marmoset, Callithrix jacchus. Laboratory Animals 23(4): 353-356, 1989

Influence of semen collection method on ejaculate characteristics in the common marmoset, Callithrix jacchus. Journal Of Andrology. 17(2): 164-172, 1996

The intersexual agonistic behavior related to food access in common marmoset Callithrix jacchus pairs O comportamento agonista intersexos em funcao do acesso ao alimento em pares de saguis Callithrix jacchus. Unknown, 1997

Non-invasive blood pressure measurement: values, problems and applicability in the common marmoset (Callithrix jacchus). Laboratory Animals 49(3): 241-250, 2015

Measuring physiological stress in the common marmoset ( Callithrix jacchus ): Validation of a salivary cortisol collection and assay technique. Physiology & Behavior 185: 14-22, 2018

Measuring physiological stress in the common marmoset (Callithrix jacchus): Validation of a salivary cortisol collection and assay technique. Physiology and Behavior 185: 14-22, 2018

Penile vibrostimulation An improved method for semen collection in the marmoset monkey. Reproduction in Domestic Animals 34(1): 41, 1999

The fertility of daughters in common marmoset callithrix jacchus jacchus family groups. Primates 30(3): 423-432, 1989

Frequency representation in auditory cortex of the common marmoset (Callithrix jacchus jacchus). Journal of Comparative Neurology 252(2): 175-185, 1986