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Novel approach to quantitative reverse transcription PCR assay of mRNA component in autopsy material using the TaqMan fluorogenic detection system: Dynamics of pulmonary surfactant apoprotein A



Novel approach to quantitative reverse transcription PCR assay of mRNA component in autopsy material using the TaqMan fluorogenic detection system: Dynamics of pulmonary surfactant apoprotein A



Forensic Science International 113(1-3): 127-131, September 11th



A novel approach to quantitative reverse transcription (RT)-PCR assay of mRNA component using fluorescent TaqMan methodology and a new instrument (ABI Prism 7700 sequence detection system) was developed for autopsy materials. Pulmonary surfactant apoprotein A (SP-A) mRNA from a cadaveric lung was quantitated in real-time. The target SP-A gene and the endogenous reference of glyceraldehyde-3-phosphate (GAPDH) were amplified in the same tube, and an amount of the target was normalized to the reference. This assay had a high reproducibility and discrimination even in forensic autopsy materials up to 96 h postmortem. Elevated SP-A expressions were determined in some cases. This system without post-PCR sample handling would be a very useful tool in pathological diagnosis and DNA analysis.

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Accession: 011071336

Download citation: RISBibTeXText

PMID: 10978613

DOI: 10.1016/s0379-0738(00)00252-8


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