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Nucleoside kinases of marine bacteria: Occurrence and properties

Nucleoside kinases of marine bacteria: Occurrence and properties

Mikrobiologiya 67(4): 496-504

Cell extracts of 26 strains from the Collection of Marine Microorganisms of the Pacific Ocean Institute of Bioorganic Chemistry were assayed for the presence of thymidine and uridine kinases. Thymidine kinase activity was revealed in all 26 of the strains tested, and uridine kinase activity was found in 22 strains. Cells with sufficiently high kinase activity rapidly consumed the respective labeled nucleoside in vivo; thymidine was incorporated into DNA, and uridine, into both DNA and RNA. Ion-exchange chromatography of cell extracts showed that each of the three strains studied in this respect contained several enzymes capable of phosphorylating thymidine, uridine, and thymidine monophosphate. Purified nucleoside kinases were examined for their ability to use various nucleosides as phosphate acceptors. It was found that all the nucleoside kinases examined had broad substrate specificity.

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Accession: 011075610

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