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PML is required for homeodomain-interacting protein kinase 2 (HIPK2) -mediated p53 phosphorylation and cell cycle arrest but is dispensable for the formation of HIPK domains

Möller, A.; Sirma, Hüseyin.; Hofmann, T.G.; Rueffer, S.; Klimczak, E.; Dröge, W.; Will, H.; Schmitz, M.Lienhard.

Cancer Research 63(15): 4310-4314

2003


ISSN/ISBN: 0008-5472
PMID: 12907596
Accession: 011111651

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Here we demonstrate that endogenous human homeodomain-interacting protein kinase (HIPK) 2 and the highly homologous kinase HIPK3 are found in a novel subnuclear domain, the HIPK domains. These are distinct from other subnuclear structures such as Cajal bodies and nucleoli and show only a partial colocalization with promyelocytic leukemia (PML) nuclear bodies (PML-NBs). A kinase inactive HIPK2 point mutant is localized in the nucleoplasm.

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