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Performance of pectolytic enzymes during hydrolysis of pectic substances under assay conditions: A statistical approach

Naidu, G.S.thya Narayana; Panda, T.

Enzyme and Microbial Technology 25(1-2): 116-124

1999


ISSN/ISBN: 0141-0229
DOI: 10.1016/s0141-0229(99)00017-4
Accession: 011129791

The reaction conditions were optimized for enzymatic hydrolysis of pectic substances using pectolytic enzymes produced by Aspergillus niger NCIM 548. Response surface methodology was used to optimize the reaction parameters and the central composite design was employed for this purpose. Pectin was used as the substrate for polymethylgalacturonase and pectinlyase, whereas polygalacturonic acid was the substrate for polygalacturonase. The volume ratio of reactants for maximum hydrolysis was determined under the assay conditions. The optimum amount of substrate and enzyme were found to be 0.2 cm3 and 0.074 cm3 for polymethylgalacturonase, 0.4 cm3 and 0.086 cm3 for polygalacturonase, and 0.63 cm3 and 0.7 cm3 for pectinlyase. The pH and temperature optima were found to be 5.3 and 30degreeC for polymethylgalacturonase, 6.6 and 26degreeC for polygalacturonase, and 4.8 and 35degreeC for pectinlyase. Under optimized conditions the enzyme activities were higher when compared to unoptimized conditions.

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