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A new species of the genus Haemogamasus from China (Acarina: Haemogamasidae)
Reference ranges for hematologic and serum biochemical values in llamas (Lama glama)
Slime mold beetles of the genus Anisotoma (Leiodidae): classification and evolution
To be young, gifted, black and female: a meditation on the cultural politics at play in representations of Venus and Serena Williams
Anti-drool cheiloplasty: clinical results in six dogs
Mentha taxonomic status as interpreted through cytology genetics and phytochemistry
Geomorphology of Lightning Creek valley, Manning Park, southwestern British Columbia
Algunos peces del Rio Autana
Development of a generic high-yield fed batch process for large-scale GMP manufacturing of human monoclonal antibodies
Treatment of 40 cases of paralysis of common peroneal nerve with acupuncture and moxibustion
Studies into the pigments in beetroot (Beta vulgaris L. ssp. vulgaris var. rubra L.)
Principles of the organization of therapeutic and preventive care in gynecologic diseases in children and adolescents
The assessment of binge eating severity among obese persons
Sample size requirements for evaluating a conservative therapy
Intensive production systems of fig (Ficus carica L.) under greenhouse conditions
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Peptic ulcer and multiple papillomas of the esophagus and pylorus in an infant
Mind as a force field: comments on a new interactionistic hypothesis
Endothelin-1 plasma concentration in children and adolescents with atherogenic risk factors
Simple and rapid extraction method of total egg lipids for determining organochlorine pesticides in the egg
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Primary duodenal tuberculosis diagnosed by endoscopic biopsy
Hypohydration and exercise: effects of heat acclimation, gender, and environment
Apropos of a Case of Esophageal Tuberculosis
Contributions to the knowledge of the Freshwater Entomostraca of South America, as shown by artificial hatching from dried material. Part i
Nutrient inputs to the Choptank River estuary: Implications for watershed management
Toxic metabolites of Amanita pantherina, A. cothurnata, A. muscaria and other Amanita species
Basic concepts and issues: a primer on distribution and sales representative agreements in the medical device and durable medical equipment industries
Recent changes in vaccination guidelines. An update on vaccines and target population initiatives
The results of research on the antioxidant emoksipin in clinical eye diseases
An overview of selected diseases and drug needs in the llama and alpaca industries
Hyperbaric oxygen therapy (HBOT) and peridontal health
Minimal Descriptors for Drumstick (Moringa oleifera Lam.) - An Underutilized Vegetable Crop
Demembrement du genre Cicindela L. (fin). V. Faune australienne. (Et liste recapitulative des genres et sous-genres proposes pour la faune mondiale)
Pollution of ground water by nutrients and fecal coliforms from lakeshore septic tank systems
Uber die Ausbruche von Maul- und Klauenseuche in den ersten 14 Tagen nach der aktiven Immunisierung mit Vakzine nach Waldmann und Kobe - Outbreaks of foot-and-mouth disease in the first 14 days after
Patologie delle alte vie respiratorie del bambino in ambulatorio: un'esperienza italiana. Diseases of the upper respiratory tract in children in ambulatory care: an Italian experience
Fundamentals of the scoliosis problem for the general practitioner
Estimating the time and temperature relationship for causation of deep-partial thickness skin burns
Containing costs in the ED
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Phorbol ester-induced activation of mitogen-activated protein kinase/extracellular-signal-regulated kinase kinase and extracellular-signal-regulated protein kinase decreases glucose-6-phosphatase gene expression


Phorbol ester-induced activation of mitogen-activated protein kinase/extracellular-signal-regulated kinase kinase and extracellular-signal-regulated protein kinase decreases glucose-6-phosphatase gene expression



Biochemical Journal 357(Pt 3): 867-873



ISSN/ISBN: 0264-6021

PMID: 11463359

DOI: 10.1042/0264-6021:3570867

Glucose-6-phosphatase (G6Pase) plays a central role in blood glucose homoeostasis, and insulin suppresses G6Pase gene expression by the activation of phosphoinositide 3-kinase (PI 3-kinase). Here, we show that the phorbol ester PMA decreases both basal and dexamethasone/cAMP-induced expression of a luciferase gene under the control of the G6Pase promoter in transiently transfected H4IIE hepatoma cells. This regulation was suppressed by the inhibitors of the mitogen-activated protein kinase/extracellular-signal-regulated kinase kinase (MEK), PD98059 and U0126, but not by the inhibitor of PI 3-kinase, LY294002. The co-expression of a constitutively active mutant of MEK mimicked the regulation of G6Pase promoter activity by PMA. The effect of PMA on both basal and induced G6Pase gene transcription was impaired by the overexpression of a dominant negative MEK construct, as well as by the expression of mitogen-activated protein kinase phosphatase-1. The mutation of the forkhead-binding sites within the insulin-response unit of the G6Pase promoter, which decreases the effect of insulin on G6Pase gene expression, did not alter the regulation of gene expression by PMA. The data show that PMA decreases G6Pase gene expression by the activation of MEK and extracellular-signal regulated protein kinase. With that, PMA mimics the effect of insulin on G6Pase gene expression by a different signalling pathway.

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