EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Platelet activation by a relapsing fever spirochaete results in enhanced bacterium-platelet interaction via integrin alphaIIbbeta3 activation


, : Platelet activation by a relapsing fever spirochaete results in enhanced bacterium-platelet interaction via integrin alphaIIbbeta3 activation. Molecular Microbiology 39(2): 330-340

Borrelia hermsii, a spirochaete responsible for relapsing fever in humans, grows to high density in the bloodstream and causes thrombocytopenia. We show here that B. hermsii binds to human platelets. Extended culture in bacteriological medium resulted in both diminished infectivity in vivo and diminished platelet binding in vitro. Platelet binding was promoted by the platelet integrin alphaIIbbeta3: the bacterium bound to purified integrin alphaIIbbeta3, and bacterial binding to platelets was diminished by alphaIIbbeta3 antagonists or by a genetic defect in this integrin. Integrin alphaIIbbeta3 undergoes a conformational change upon platelet activation, and bacteria bound more efficiently to activated rather than resting platelets. Nevertheless, B. hermsii bound at detectable levels to preparations of resting platelets. The bacterium did not recognize a point mutant of alphaIIbbeta3 that cannot acquire an active conformation. Rather, B. hermsii was capable of triggering platelet and integrin alphaIIbbeta3 activation, as indicated by the expression of the platelet activation marker P-selectin and integrin alphaIIbbeta3 in its active conformation. The degree of platelet activation varied depending upon bacterial strain and growth conditions. Prostacyclin I2, an inhibitor of platelet activation, diminished bacterial attachment, indicating that activation enhanced bacterial binding. Thus, B. hermsii signals the host cell to activate a critical receptor for the bacterium, thereby promoting high-level bacterial attachment.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 011160755

PMID: 11136454

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Engelhardt, R., 2005: Developing local capacities for conserving heritage resource and managing tourism at heritage sites. This paper describes the implementation of the UNESCO project on "Cultural Heritage Management and Tourism: Models for Co-operation Among Stakeholders" (1998-2003). The project aimed to provide workable solutions to find a common ground...

Walkowiak, J.; Krawczynski, M.; Herzig, K-H., 2004: Giardiasis aggravates malabsorption in cystic fibrosis. Persistent steatorrhoea was observed in 3 pancreatic-insufficient, malnourished patients with cystic fibrosis (2-3 years old) [Poland]. Partial villous atrophy and Giardia intestinalis were detected on histopathological examination of intestinal s...

Germain, Louis., 1913: Contribution a la faune malacologique de Madagascar. I. Le genre Clavator. Bulletin du Museum Paris, 473-477

Chebotarev, N. T.; Bubnova, V. N., 2008: Effect of the long-term application of mineral fertilizers and lime on the reproduction of fertility and productivity of soddy-podzolic soil. The aftereffect of lime applied separately (for neutralizing 1.0 and 2.0 potential acidity) and in combination with NPK for compensating the removal of nutrients with perennial grasses was studied in a field stationary C experiment on loamy soddy...

Akinin Vyacheslav; Miller Elizabeth; Mukasa Samuel B., 2004: Reworking of deep crust; records from lower to middle crust xenoliths of the Bering Sea region and northeastern Siberia. International Geological Congress, Abstracts = Congres Geologique International, Resumes 32, Part 1: 761

Krivolutskii, D. A., 1965: A new finding of Opilioacarina in the USSR. A new representative of the group Opilioacarina is found in the litter of walnut-fruit forest near the town of Tashkent. Only one finding of the representatives of this order was previously known in USSR.

Rayss, T., 1951: Nouvelle contribution a la connaissance des Uredinees de Palestine. Nearly 200 spp. of plant rusts are known to occur in Palestine. Puccinia rimosa f. nattrassi on Juncus maritimus and Uromyces anthyllidis f. trigoneillae on Trigonella are descr. 100 spp., mostly abundantly annotated, are reported. Extensive bibli...

Kerekes J., 1988: Faunistic studies on epigeic spider community on sandy grassland. As a result of the experiment on the sandy grassland of Kiskunsag National Park between 1979-85 139 species of 20 Araneidea families have been defined. Tarentula fabrilis (Clerc, 1758) (Lycosidae) out of them is new to Hungary. This paper contains...

Benito, M.; Masaguer, A.; De Antonio, R.; Moliner, A., 2004: Use of pruning waste compost as a component in soilless growing media. The objective of this work was to study the use of pruning wastes compost (PWC) as a growing media component for ornamental plants. The main physical, chemical and biological characteristics of PWC were analysed in order to evaluate its suitabilit...

Shishkin Mikhail Aleksandrovich, 1990: Concerning the tripartite subdivision of the upper Tatarian substage of the Upper Permian based on the fauna of terrestrial vertebrates. Byulleten' Moskovskogo Obshchestva Ispytateley Prirody, Otdel Geologicheskiy 65(2): 117