+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Pore network modelling of affinity chromatography: determination of the dynamic profiles of the pore diffusivity of beta-galactosidase and its effect on column performance as the loading of beta-galactosidase onto anti-beta-galactosidase varies with time



Pore network modelling of affinity chromatography: determination of the dynamic profiles of the pore diffusivity of beta-galactosidase and its effect on column performance as the loading of beta-galactosidase onto anti-beta-galactosidase varies with time



Journal of Biochemical and Biophysical Methods 49(1-3): 123-139



A three-dimensional pore network model for diffusion in porous adsorbent particles was employed in a dynamic adsorption model that simulates the adsorption of a solute in porous particles packed in a chromatographic column. The solution of the combined model yielded the dynamic profiles of the pore diffusion coefficient of beta-galactosidase along the radius of porous adsorbent particles and along the length of the column as the loading of beta-galactosidase onto anti-beta-galactosidase immobilized on the surface of the pores of the particles occurred, and, the dynamic adsorptive capacity of the chromatographic column as a function of the design and operational parameters of the chromatographic system. It was found that for a given column length the dynamic profiles of the pore diffusion coefficient were influenced by (a) the superficial fluid velocity in the column, (b) the diameter of the adsorbent particles, and (c) the pore connectivity of the porous structure of the adsorbent particles. The effect of the magnitude of the pore connectivity on the dynamic profiles of the pore diffusion coefficient of beta-galactosidase increased as the diameter of the adsorbent particles and the superficial fluid velocity in the column increased. The dynamic adsorptive capacity of the column increased as (i) the particle diameter and the superficial fluid velocity in the column decreased, and (ii) the column length and the pore connectivity increased. In preparative affinity chromatography, it is desirable to obtain high throughputs within acceptable pressure gradients, and this may require the employment of larger diameter adsorbent particles. In such a case, longer column lengths satisfying acceptable pressure gradients with adsorbent particles having higher pore connectivity values could provide high dynamic adsorptive capacities. An alternative chromatographic system could be comprised of a long column packed with large particles which have fractal pores (fractal particles) that have high pore connectivities and which allow high intraparticle diffusional and convective flow mass transfer rates providing high throughputs and high dynamic adsorptive capacities. If large scale monoliths could be made to be reproducible and operationally stable, they could also offer an alternative mode of operation that could provide high throughputs and high dynamic adsorptive capacities.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 011170035

Download citation: RISBibTeXText

PMID: 11694276


Related references

Pore network modelling: determination of the dynamic profiles of the pore diffusivity and its effect on column performance as the loading of the solute in the adsorbed phase varies with time. Journal of Chromatography. a 908(1-2): 35-47, 2001

Use of inactive beta-D-galactosidase for elimination of interference by anti-beta-D-galactosidase antibodies in immune complex transfer enzyme immunoassay for anti-thyroglobulin IgG in serum using beta-D-galactosidase from Escherichia coli as label. Journal of Clinical Laboratory Analysis 5(3): 197-205, 1991

Affinity chromatography of beta-galactosidase on controlled-pore glass derivatives. Journal of Chromatography 104(1): 105-111, 1975

Comparison of activities of acid beta -galactosidase, beta -glucuronidase, N-acetyl- beta -glucosaminidase and alpha -galactosidase in jejunum and ileum of adult and suckling rats. Comparative Biochemistry and Physiology 43B(1): 1-8, 1972

A sensitive micro-immunoassay using beta-galactosidase/anti-beta-galactosidase complexes. Journal of Immunological Methods 97(1): 19-27, 1987

A sensitive micro immunoassay using beta galactosidase anti beta galactosidase complex. Journal of Immunological Methods 97(1): 19-28, 1987

Beta -Galactosidase activity of the jejunum and ileum of suckling rats. Comparison of activities of beta -galactosidase at different concentration of substrates (o-nitrophenyl- beta -D-galac-toside and lactose) at different pH. Biologia Neonat 10: 5/6, 241-53, 1966

Production of beta -galactosidase by Kluyveromyces marxianus MTCC 1388 using whey and effect of four different methods of enzyme extraction on beta -galactosidase activity. Indian Journal Of Microbiology: 3, 337-341, 2008

Studies on .BETA.-galactosidase. II. Purificaof .BETA.-of .BETA.-galactosidase from Macrophomina phaseoli and its enzymatic properties. Chemical & Pharmaceutical Bulletin 24(4): 794-799, 1976

Alternative splicing of beta-galactosidase mRNA generates the classic lysosomal enzyme and a beta-galactosidase-related protein. Journal of Biological Chemistry 264(34): 20655-20663, 1989

Immobilized beta galactosidase ec 3.2.1.23 on insoluble carrier part 1 immobilization and properties of escherichia coli beta galactosidase. Lebensmittel-Wissenschaft & Technologie 8(6): 274-277, 1975

Differential induction of beta galactosidase and phospho beta galactosidase activities in the fermentation of whey permeate by clostridium acetobutylicum. Applied Microbiology & Biotechnology 26(3): 254-257, 1987

Reduction of lactose content of milk by entrapped beta-galactosidase. I. Characteristics of beta-galactosidase from yeast and Escherichia coli. Journal of Dairy Science 56(9): 1123-1127, 1973

Galactosialidosis (beta-galactosidase-neuraminidase deficiency): a possible role of serine-thiol proteases in the degradation of beta-galactosidase molecules. Clinica Chimica Acta; International Journal of Clinical Chemistry 125(3): 275-282, 1982

Immobilization of beta -galactosidase on an inert support and examination of the properties of the immobilized enzyme. II. Technological applications of beta -galactosidase. Lebensmittel Wissenschaft + Technologie 8(6): 278-281, 1975