+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Postnatal development of inhibitory synaptic transmission in the rostral nucleus of the solitary tract



Postnatal development of inhibitory synaptic transmission in the rostral nucleus of the solitary tract



Journal of Neurophysiology 85(5): 2203-2212



To explore the postnatal development of inhibitory synaptic activity in the rostral (gustatory) nucleus of the solitary tract (rNST), whole cell and gramicidin perforated patch-clamp recordings were made in five age groups of rats [postnatal day 0-7 (P0-7), P8-14, P15-21, P22-30, and P >55]. The passive membrane properties of the developing rNST neurons as well as the electrophysiological and pharmacological characteristics of single and tetanic stimulus-evoked inhibitory postsynaptic potentials (IPSPs) were studied in brain slices under glutamate receptor blockade. During the first postnatal weeks, significant changes in resting membrane potential, spontaneous activity, input resistance, and neuron membrane time constant of the rNST neurons occurred. Although all the IPSPs recorded were hyperpolarizing, the rise and decay time constants of the single stimulus shock-evoked IPSPs decreased, and the inhibition response-concentration function to the gamma-aminobutyric acid (GABA) receptor antagonist bicuculline methiodide (BMI) shifted to the left during development. In P0-7 and P8-14, but not in older animals, the IPSPs had a BMI-insensitive component that was sensitive to block by picrotoxin, suggesting a transient expression of GABA(C) receptors. Tetanic stimulation resulted in both short- and long-term changes of inhibitory synaptic transmission in the rNST. For P0-7 and P8-14 animals tetanic stimulation resulted in a sustained hyperpolarization that was maintained for some time after termination of the tetanic stimulation. In contrast, tetanic stimulation of neurons in P15-21 and older animals resulted in hyperpolarization that was not sustained but decayed back to a more positive level with an exponential time course. Tetanic stimulation resulted in potentiation of single stimulus shock-evoked IPSPs in ~50% of neurons in all age groups. These developmental changes in inhibitory synaptic transmission in the rNST may play an important role in shaping synaptic activity in early development of the rat gustatory system during a time of maturation of taste preferences and aversions.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 011174366

Download citation: RISBibTeXText

PMID: 11353035


Related references

Postnatal Development of Inhibitory Synaptic Transmission in the Rostral Nucleus of the Solitary Tract. Journal of Neurophysiology 85(5): 2203-2212, 2001

Potentiation of GABAergic synaptic transmission in the rostral nucleus of the solitary tract. Neuroscience 94(4): 1173-1182, 1999

Potentiation of GABAergic synaptic transmission in the rostral nucleus of the solitary tract. Neuroscience 94(4): 1173-1182, 1999

Excitatory and inhibitory synaptic function in the rostral nucleus of the solitary tract in embryonic rat. Brain Research 1490: 117-127, 2013

Time course of GABA in the synaptic clefts of inhibitory synapses in the rostral nucleus of the solitary tract. Neuroscience Letters 373(1): 10-15, 2005

In vitro study of afferent synaptic transmission in the rostral gustatory zone of the rat nucleus of the solitary tract. Brain Research. 702(1-2): 188-198, 1995

Tetanic Stimulation Induces Short-Term Potentiation of Inhibitory Synaptic Activity in the Rostral Nucleus of the Solitary Tract. Journal of Neurophysiology 79(2): 595-604, 1998

Tetanic stimulation induces short-term potentiation of inhibitory synaptic activity in the rostral nucleus of the solitary tract. Journal of Neurophysiology 79(2): 595-604, 1998

Activation of p2x receptors facilitates excitatory, but not inhibitory, synaptic transmission in the nucleus of the solitary tract of the rat. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 549 13, 2002

Isoflurane differentially modulates inhibitory and excitatory synaptic transmission to the solitary tract nucleus. Anesthesiology 108(4): 675-683, 2008

Effects of early postnatal receptor damage on dendritic development in gustatory recipient zones of the rostral nucleus of the solitary tract. Developmental Brain Research 61(2): 197-206, 1991

Presynaptic actions of propofol enhance inhibitory synaptic transmission in isolated solitary tract nucleus neurons. Brain Research 1286: 75-83, 2009

Frequency-dependent properties of inhibitory synapses in the rostral nucleus of the solitary tract. Journal of Neurophysiology 89(1): 199-211, 2003

Synaptic interactions due to convergent input from gustatory afferent fibers in the rostral nucleus of the solitary tract. Journal of Neurophysiology 76(5): 2919-2927, 1996

Pre- and postnatal differences in membrane, action potential, and ion channel properties of rostral nucleus of the solitary tract neurons. Journal of Neurophysiology 106(5): 2709-2719, 2011