+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Postoperative exposure to glove powders modulates production of peritoneal eicosanoids during peritoneal wound healing



Postoperative exposure to glove powders modulates production of peritoneal eicosanoids during peritoneal wound healing



European Journal of Surgery 165(7): 698-704



To assess the effect of postsurgical exposure of peritoneal cavity to glove powders, Hydrocote, latex proteins, and lipopolysaccharide (LPS) on eicosanoid production in peritoneal fluid and cellular distribution of eicosanoid enzymes in peritoneal wound during healing. Randomised experimental study. Institute for Wound Research, USA. 360 mice randomised into six groups of 60 each. Abrasion of peritoneal cavity followed by instillation of 500 microl of sterile phosphate buffered saline (PBS) alone (Control) or containing 100 microg/ml of Biosorb, Keoflo, Hydrocote, 1 mg/ml of latex proteins, or 12.5 microg/ml of LPS. Mice were killed at 1, 2, 4, 7, 14 and 28 days, and the peritoneal washing obtained from each animal and concentration of eicosanoids measured. Tissue were immunostained for cyclooxygenases and 5-lipoxygenase and thromboxane A2 (TXA2) synthetase. Peritoneal fluid from uninjured controls contained 3.9 (0.8), 5.2 (0.3) and 0.2 (0.02) ng/ml of thromboxane B2 (TXB2), prostaglandin E2 (PGE2) and leukotriene B4 (LTB4), respectively. These increased significantly during the first week to 6.3 (0.3), 11.7 (0.8) and 2.6 (0.1) ng/ml, p<0.05, before returning to baseline by day 14. In all the treated groups the values were significantly higher than in controls (p<0.05). Immunoreactive cyclo-oxygenases, 5-lipoxygenase and TXA2 synthetase proteins were present in various cell types in uninjured skin and peritoneum, incisional and peritoneal wounds and adhesion tissues. Staining was more intense at the site of wounds and paralleled eicosanoid concentrations during healing. There was no difference between exposed and unexposed groups. The presence of glove powders, latex proteins and LPS in peritoneal cavity cause increased eicosanoid production and aggravate the normal inflammatory reaction to tissue injury. This may contribute to the inflammatory or immune reactions and development of adhesions caused by glove powders.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011174721

Download citation: RISBibTeXText

PMID: 10452266

DOI: 10.1080/11024159950189771


Related references

The effect of postoperative peritoneal lavage on survival, peritoneal wound healing and adhesion formation following fecal peritonitis: an experimental study in the rat. British Journal of Surgery 61(8): 601-604, 1974

Postsurgical intraperitoneal exposure to glove powders modulates inflammatory and immune-related cytokine production. Wound Repair and Regeneration 5(1): 89-96, 1997

The effect of protein deficiency upon peritoneal healing and peritoneal wound contraction in therat. Brit. J. Nutrition. 23: 497-504, 1969

Response of the human peritoneal mesothelial cell to injury: an in vitro model of peritoneal wound healing. Kidney International 54(6): 2160-2169, 1998

Effects of postoperative intraperitoneal chemotherapy on peritoneal wound healing and adhesion formation. Cancer Treatment and Research 82: 327-335, 1996

Response of human peritoneal mesothelial cells to injury An in vitro model to study wound healing during peritoneal dialysis. Journal of the American Society of Nephrology 9(PROGRAM AND ABSTR ISSUE): 530A, 1997

An integrin-activating peptide, PHSRN, ameliorates inhibitory effects of conventional peritoneal dialysis fluids on peritoneal wound healing. Nephrology Dialysis Transplantation 25(4): 1109-1119, 2010

Surgical glove powders differentially modulate macrophage and lymphocyte-derived cytokines and eicosanoids production in vitro. Wound Repair and Regeneration 3(4): 518-526, 1995

Effect of peritoneal lavage with povine-iodine on prevention of postoperative sepsis after exposure of peritoneal cavity to sea water in rat. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 16(2): 103-105, 2004

Increased peritoneal protein permeability in continuous ambulatory peritoneal dialysis associated peritonitis by eicosanoids. Kidney International 29(1): 237, 1986

Peritoneal dialysis effluent inhibits the release of cytokines and eicosanoids from human peritoneal macrophages pmo. Journal of the American Society of Nephrology 3(3): 420, 1992

Time of peritoneal cavity exposure influences postoperative glucose production. Canadian Journal of Anaesthesia 46(4): 352-358, 1999

The impairment of wound healing process is correlated with abnormalities of TNF-alpha production by peritoneal exudate cells in obstructive jaundiced rats. Hpb Surgery 11(5): 311-318, 2000

Effect of peritoneal effluent after 2 months of daily peritoneal dialysate administrations on TNF-alpha production by normal rat peritoneal macrophages. Nephrology Dialysis Transplantation 15(9): A231, 2000