Pregnancy following transfer of cryopreserved-thawed embryos that had been a result of fertilization of all in vitro matured metaphase or germinal stage oocytes. case report
Check, M.L.; Brittingham, D.; Check, J.H.; Choe, J.K.
Clinical and Experimental Obstetrics and Gynecology 28(2): 69-70
Purpose: To see if pregnancies can be achieved after performing intracytoplasmic sperm injection (ICSI) of in vitro matured metaphase I or germinal vesicle stage oocytes and then cryopreserving them at the 2 pronuclear stage. Methods: Transfer of frozen/thawed hatched day 3 embryos to two women. All embryos were a result of in vitro maturation of immature oocytes followed by ICSI. Results: Both women conceived. One has delivered and one has successfully completed the first trimester. Conclusion: These two cases represent only the second and third reported cases of pregnancies following frozen embryo transfer where the embryos resulted from fertilizing immature oocytes by ICSI.