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Preparative isolation and purification of phenylethanoid glycosides from the extract of faeces of beagle dogs by high-speed countercurrent chromatography



Preparative isolation and purification of phenylethanoid glycosides from the extract of faeces of beagle dogs by high-speed countercurrent chromatography



Journal of Liquid Chromatography & Related Technologies 24(14): 2187-2195



High-speed countercurrent chromatography (HSCCC) was applied to the separation of acteoside and isoacteoside from a crude extract from canine faeces after the dogs were orally administered phenylethanoid glycosides (PhGs). Two-step separation with a quaternary two-phase solvent system composed of ethyl acetate-n-butanol-ethanol-water (system 1, 35:6:6:50, v/v; system 2, 30:10:6:50, v/v) was used. HPLC analysis of final CCC fractions revealed that the two main PhGs showed purity of over 95%. Their chemical structures were identified by 1HNMR, 13CNMR, FAB-MS.

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Accession: 011189590

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DOI: 10.1081/jlc-100104901


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