+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Preventive effect of indomethacin and melatonin on 7,12-dimethylbenz/a/anthracene-induced mammary carcinogenesis in female Sprague-Dawley rats. A preliminary report

Preventive effect of indomethacin and melatonin on 7,12-dimethylbenz/a/anthracene-induced mammary carcinogenesis in female Sprague-Dawley rats. A preliminary report

Folia Biologica 47(2): 75-79

The aim of the experiment was to analyse the oncostatic effect of nonsteroidal antiinflammatory drug INDO, hormone MEL and combination of both substances in DMBA-induced mammary carcinogenesis in female SD rats. Chemoprevention started 10 days before the application of the first dose of DMBA to 35-day-old rats. INDO was administered in tap water (20 mug/ml of water) for 3 days in a week (days 2, 4 and 6), MEL solution in the concentration of 20 mug/ml of tap water was administered between 3 p.m. and 8 a.m. for 4 days in a week (days 1, 3, 5 and 7); during other days the animals drank tap water only. In combined chemoprevention, rats were drinking solutions of INDO and MEL according to the above-mentioned scheme. DMBA in the dose of 10 mg/rat was administered intragastrically using a probe to all rats 3 times on postnatal days 45, 50 and 55. There were four experimental groups: group 1 - without chemoprevention, group 2 - INDO treatment, group 3 - MEL treatment, group 4 - application of INDO + MEL. The experiment lasted 26 weeks from the first administration of DMBA, when the final incidence and frequency of tumours per animal and group, as well as latency and average volume of tumours were evaluated. The content/concentration of malondialdehyde (MDA) was determined in selected tissues as a criterion of lipoperoxidation, considering its potential influencing by chemoprevention. The tumour incidence in controls was 100%; INDO reduced the incidence (36.84%) and frequency per group and animal, decreased the mean volume of tumours and prolonged the latency. Chemoprevention using combination of INDO with MEL was successful like that with INDO; however, it did not influence the tumour volume. MEL decreased the incidence to 42.11% and pronouncedly reduced the tumour frequency per group. INDO, administered alone or in combination with MEL, reduced an increased content/concentration of MDA in the liver, bone marrow and serum of tumour-bearing rats. INDO, MEL and INDO + MEL had a pronounced chemopreventive effect and showed to be a favourable combination in prevention of experimental mammary carcinogenesis.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 011196469

Download citation: RISBibTeXText

PMID: 11321252

Related references

Preventive and curative effect of melatonin on mammary carcinogenesis induced by dimethylbenz[a]anthracene in the female Sprague-Dawley rat. Breast Cancer Research 7(4): R470-R476, 2005

Phenoxodiol, a novel isoflavone derivative, inhibits dimethylbenz[a]anthracene (DMBA)-induced mammary carcinogenesis in female Sprague-Dawley rats. European Journal of Cancer 39(7): 1012-1018, 2003

Effects of tamoxifen and melatonin on mammary gland cancer induced by N-methyl-N-nitrosourea and by 7,12-dimethylbenz(a)anthracene, respectively, in female Sprague-Dawley rats. Folia Biologica 47(1): 5-10, 2001

Preventive effect of Morinda citrifolia at the initiation stage of mammary breast carcinogenesis induced by 7,12-dimethylbenzo anthracene in female Sprague-Dawley rats. Cancer Epidemiology Biomarkers & Prevention 11(10 Part 2): 1218s, 2002

Dose response chemopreventive potential of allyl isothiocyanate against 7,12-dimethylbenz(a)anthracene induced mammary carcinogenesis in female Sprague-Dawley rats. Chemico-Biological Interactions 231: 35-43, 2015

Post-initiation inhibitory effects of green tea catechins on 7,12-dimethylbenz[a]anthracene-induced mammary gland carcinogenesis in female Sprague-Dawley rats. Cancer Letters 116(1): 47-52, 1997

Promotion of dimethylbenz[a]anthracene-initiated mammary carcinogenesis by iron in female Sprague-Dawley rats. Carcinogenesis 18(9): 1757-1762, 1997

Lack of chemopreventive effects of alpha-eleostearic acid on 7,12-dimethylbenz[a]anthracene (DMBA) and 1,2-dimethylhydrazine (DMH)-induced mammary and colon carcinogenesis in female Sprague-Dawley rats. Food and Chemical Toxicology 44(2): 271-277, 2006

Effect of neonatal administration of sex steroids on 7,12-dimethylbenz[a]anthracene-induced mammary carcinoma and dysplasia in female Sprague-Dawley rats. Gan 69(5): 627-631, 1978

Polyunsaturated fatty acids as promoters of mammary carcinogenesis induced in Sprague-Dawley rats by 7,12-dimethylbenz[a]anthracene. Journal of the National Cancer Institute 66(3): 517-522, 1981

Chemopreventive potential of ferulic acid in 7,12-dimethylbenz[a]anthracene-induced mammary carcinogenesis in Sprague-Dawley rats. European Journal of Pharmacology 637(1-3): 22-29, 2010

Long term inhibition by Estradiol or Progesterone of Melatonin secretion after administration of a mammary carcinogen, the dimethyl benz(a)anthracene, in Sprague-Dawley female rat; inhibitory effect of Melatonin on mammary carcinogenesis. Breast Cancer Research and Treatment 79(3): 365-377, 2003

Seasonal influence on 7,12-dimethylbenz[a]anthracene-induced mammary carcinogenesis in Sprague-Dawley rats under controlled laboratory conditions. Pharmacology and Toxicology 81(6): 265-270, 1997

Chemopreventive potential of fungal taxol against 7, 12-dimethylbenz[a]anthracene induced mammary gland carcinogenesis in Sprague Dawley rats. European Journal of Pharmacology 767: 108-118, 2015

Inhibition of mammary gland carcinogenesis by green tea catechins and other naturally occurring antioxidants in female Sprague-Dawley rats pretreated with 7,12-dimethylbenz[alpha]anthracene. Cancer Letters 83(1-2): 149-156, 1994