+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Production of cutinase by Thermomonospora fusca ATCC 27730

Production of cutinase by Thermomonospora fusca ATCC 27730

Journal of applied microbiology 86(4): 561-568

Ten strains belonging to various Thermomonospora species were tested for their ability to hydrolyse the insoluble plant polyester cutin. One strain, the thermophile T. fusca ATCC 27730, was found to produce a highly inducible cutinase when grown in broth medium containing purified apple cv. Golden Delicious cutin. Apple pomace, tomato peel, potato suberin and commercial cork were also shown to induce cutinase production. Addition of glucose to the culture medium either at the beginning of fermentation or after 2 days of incubation in the presence of apple cutin led to repression of cutinase production. The cutinase was active against a wide range of cutins, including those isolated from other apple cultivars as well as tomato, cucumber, grapefruit, and green pepper. Cutinase activity in the induced culture supernatant fluids exhibited a half-life of over 60 min at 70 degrees C and a pH optimum of 11.0.Some potential applications for cutinases are discussed.

Accession: 011203486

Download citation: RISBibTeXText

DOI: 10.1046/j.1365-2672.1999.00690.x

Download PDF Full Text: Production of cutinase by Thermomonospora fusca ATCC 27730

Related references

Effect of various compounds on enkephalin hydrolysis by an aminopeptidase from the thermophiles Thermomonospora fusca ATCC 27730 and Thermus thermophilus ATCC 27634. Research Communications in Chemical Pathology and Pharmacology 41(1): 149-155, 1983

Production of a polyester degrading extracellular hydrolase from Thermomonospora fusca. Biotechnology Progress 18(5): 927-934, 2002

Production of thermostable xylanases in batch and continuous culture by thermomonospora fusca kw 3. Applied Microbiology & Biotechnology 37(4): 416-419, 1992

Fed-batch production of Thermomonospora fusca endoglucanase by recombinant Streptomyces lividans. Biotechnology & Bioengineering. 60(1): 70-76,. 5, 1998

Production of extracellular enzymes during the solubilisation of straw by Thermomonospora fusca BD25. Applied microbiology and biotechnology 41(3): 366-372, 1994

Effect of agitational shear on growth and protease production by Thermomonospora fusca. Biotechnology and Bioengineering 37(4): 371-374, 1991

Production of extracellular lignocellulose degrading enzymes by Thermomonospora fusca BD25. Biochemical Society Transactions 24(3): 378s-378s, 1996

Fed-batch production of thermomonospora fusca endoglucanase by recombinant streptomyces lividans. Biotechnology and Bioengineering 60(1): 70-76, 1999

Influence of carbon source on production of a heat stable protease from thermomonospora fusca yx. Applied Microbiology & Biotechnology 28(1): 80-84, 1988

Production of microbial protein from waste cellulose by Thermomonospora fusca, a thermophilic actinomycete. Biotechnology and Bioengineering 15(5): 833-843, 1973

Cutinase production from short-chain organic acids by Thermobifida fusca. Sheng Wu Gong Cheng Xue Bao 24(5): 821-828, 2008

Enhanced cutinase production with Thermobifida fusca by two-stage pH control strategy. Biotechnology Journal 2(3): 365-369, 2007

Optimization of extracellular lignocellulolytic enzyme production by a thermophilic actinomycete Thermomonospora fusca BD25. Enzyme & Microbial Technology 25(1-2): 38-47, July 15, 1999

Optimisation of production of extracellular non-haem peroxidases by Thermomonospora fusca BD25 in aerobic bio-reactor conditions. Biochemical Society Transactions 25(1): 65s-65s, 1997

Study on improvement of extracellular production of recombinant Thermobifida fusca cutinase by Escherichia coli. Applied Biochemistry and Biotechnology 165(2): 666-675, 2012