+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Prognostic significance of proliferative activity, DNA-ploidy, p53 and Ki-ras point mutations in colorectal liver metastases



Prognostic significance of proliferative activity, DNA-ploidy, p53 and Ki-ras point mutations in colorectal liver metastases



Cell Proliferation 31(3-4): 139-153



Paired colorectal liver metastases (CLM) and normal tissue samples from a consecutive series of 36 patients were studied prospectively. MIB-1 expression was studied by immunohistochemistry on paraffin-embedded sections. DNA ploidy and S-phase fraction (SPF) measurements were performed by flow cytometry on frozen tissues. Mutations within the p53 (exons 5-8) and c-Ki-ras (codons 12 and 13) genes were detected by PCR single-strand conformation polymorphism analysis followed by sequencing. A high correlation was observed between the MIB-1 LI and SPF value (rho=0.81; P<0.01). Moreover, p53 gene mutations were associated with either high MIB-1 LI and high SPF. In univariate analysis, SPF and MIB-1 levels were related to risk of death. The association between overall survival and DNA-ploidy or p53 mutations did not reach statistical significance, but a slightly better survival was observed for patients either with DNA-diploid tumours or without mutations (P=0.05 and P=0.06, respectively). SPF was shown by multivariate Cox model analysis to be an independent prognostic variable and thus it might be a useful prognostic factor in patients with CLM.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011206935

Download citation: RISBibTeXText

PMID: 9853427

DOI: 10.1111/j.1365-2184.1998.tb01192.x


Related references

Fas/CD95 signaling rather than angiogenesis or proliferative activity is a useful prognostic factor in patients with resected liver metastases from colorectal cancer. International Journal of Colorectal Disease 20(6): 477-484, 2005

Prognostic significance of nuclear dna ploidy patterns in resected hepatic metastases from colorectal carcinoma. Surgery (St Louis) 102(4): 635-643, 1987

Prognostic implications of proliferative activity and dna ploidy in colorectal adenocarcinoma an ecog study. Proceedings of the American Association for Cancer Research Annual Meeting 33: 217, 1992

Prognostic significance of nuclear deoxyribonucleic acid ploidy patterns in resected hepatic metastases from colorectal carcinoma. Surgery 102(4): 635-643, 1987

Prognostic significance of ploidy and proliferative activity in human malignant gliomas. International Journal of Biological Markers 8(1): 45-46, 1993

Prognostic significance of proliferative activity and ploidy in node-negative breast cancers. Annals of Oncology 4(3): 213-219, 1993

Poorly differentiated clusters in colorectal liver metastases: Prognostic significance in synchronous and metachronous metastases. Journal of Surgical Oncology 117(8): 1856-1857, 2018

The prognostic significance of lymphatics in colorectal liver metastases. Hpb Surgery 2014: 954604, 2014

Prognostic Role of BRAF Mutations in Colorectal Cancer Liver Metastases. Anticancer Research 36(9): 4805-4811, 2017

Not all point mutations are the same: A multi-institutional study of Kras in colorectal liver metastases. Hpb 21: S7-S8, 2019

Relationship between KRAS mutations and dual time point 18 F-FDG PET/CT imaging in colorectal liver metastases. Abdominal Radiology 2018, 2018

Prognostic significance of 18 F-FDG PET/CT in patients with colorectal cancer liver metastases after hepatectomy. European Journal of Surgical Oncology 44(5): 670-676, 2018

Lack of point mutations in exons 11-23 of the retinoblastoma susceptibility gene RB-1 in liver metastases of colorectal carcinoma. Oncology 59(4): 344-346, 2000

Prognostic factors after resection of colorectal liver metastases following preoperative second-line chemotherapy: Impact of RAS mutations. European Journal of Surgical Oncology 42(9): 1378-1384, 2017

Prognostic significance of histological categorization of desmoplastic reaction in colorectal liver metastases. Virchows Archiv 2019, 2019