+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Prolonged activation of transcription factor AP-1 during NGF-mediated rescue from apoptotic cell death in PC12 cells



Prolonged activation of transcription factor AP-1 during NGF-mediated rescue from apoptotic cell death in PC12 cells



Neurochemical Research 24(11): 1431-1441



Rat pheochromocytoma (PC12) cells exhibit apoptotic cell death when deprived of serum and can be rescued by nerve growth factor (NGF). We characterized AP-1 DNA binding activity in PC12 cells after serum deprivation in the presence or absence of NGF or other neurotrophic agents. There was a decline in AP-1 DNA binding activity concomitant with apoptosis in PC12 cells after serum deprivation. Treatment of serum-deprived PC12 with NGF induced persistent AP-1 binding activity that was blocked by the Trk receptor inhibitor K252a. PC12 cells treated with dibutyryl cyclic AMP or insulin also displayed increased AP-1 DNA binding activity. While NGF somewhat increased c-Fos and c-Jun protein levels transiently, it had a more robust and persistent stimulatory effect on Jun B protein levels. AP-1 transcriptional activity increased after NGF, dibutyryl cAMP, or insulin treatment under serum free conditions. Curcumin, which inhibits AP-1 activity, blocked the NGF-mediated rescue. These results wou ld suggest that the rescue of serum-deprived PC12 cells from apoptosis requires increasing endogenous levels of specific Fos/Jun components of AP-1.

(PDF emailed within 0-6 h: $19.90)

Accession: 011209774

Download citation: RISBibTeXText

PMID: 10555784

DOI: 10.1023/a:1022540925099


Related references

Apoptotic cell death and CPP32-like activation induced by thapsigargin and their prevention by nerve growth factor in PC12 cells. Biochimica Et Biophysica Acta. 140(1): 63-71,. 2, 1998

Trehalose alleviates PC12 neuronal death mediated by lipopolysaccharide-stimulated BV-2 cells via inhibiting nuclear transcription factor NF-κB and AP-1 activation. Neurotoxicity Research 26(4): 430-439, 2015

Apoptotic cell death and caspase 3 (CPP32) activation induced by calcium ionophore at low concentrations and their prevention by nerve growth factor in PC12 cells. European Journal of Biochemistry 249(1): 8-12, 1997

Multiple agents rescue Pc12 cells from serum-free cell death by translation- and transcription-independent mechanisms. The Journal of Neuroscience 11(8): 2552-2563, 1991

Multiple agents rescue PC12 cells from serum-free cell death by translation- and transcription-independent mechanisms. Journal of Neuroscience 11(8): 2552-2563, 1991

Nerve growth factor and epidermal growth factor rescue PC12 cells from programmed cell death induced by etoposide: Distinct modes of protection against cell death by growth factors and a protein-synthesis inhibitor. Neuroscience Letters 176(2): 161-164, 1994

Activation of ras and protection from apoptotic cell death by BDNF in PC12 cells expressing TrkB. Cellular Signalling. 8(5): 365-370, 1996

IGF-1 induced phosphorylation of the forkhead family of transcription factor FKHRL1 is mediated by Akt kinase, an anti-apoptotic enzyme, in PC12 cells and primary cultured neurons. Society for Neuroscience Abstracts 26(1-2): Abstract No -149 19, 2000

Insulin-like growth factor I-mediated activation of the transcription factor cAMP response element-binding protein in PC12 cells. Involvement of p38 mitogen-activated protein kinase-mediated pathway. Journal of Biological Chemistry 274(5): 2829-2837, 1999

V-CRK, an effector of the nerve growth factor signaling pathway, delays apoptotic cell death in neurotrophin-deprived PC12 cells. Biochemical Society Transactions 24(4): 573S, 1996

Cocaine induces cell death and activates the transcription nuclear factor kappa-B in PC12 cells. Molecular Brain 2: 3, 2011

Autologous dendritic cells or cells expressing both B7-1 and MUC1 can rescue tumor-specific cytotoxic T lymphocytes from MUC1-mediated apoptotic cell death. Journal of Leukocyte Biology 68(2): 225-232, 2000

Transcription factor DNA-binding activity in PC12 cells undergoing glucose deprivation-induced cell death. Society for Neuroscience Abstracts 20(1-2): 681, 1994

Zn2+-induced ERK activation mediated by reactive oxygen species causes cell death in differentiated PC12 cells. Journal of Neurochemistry 78(3): 600-610, 2001

Activation of AP-1 and nuclear factor-kappaB transcription factors is involved in hydrogen peroxide-induced apoptotic cell death of oligodendrocytes. Journal of Neurochemistry 73(6): 2501-2509, 1999