EurekaMag.com logo
+ Site Statistics
References:
53,517,315
Abstracts:
29,339,501
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Pronounced hepatic free radical formation precedes pathological liver injury in ethanol-fed rats



Pronounced hepatic free radical formation precedes pathological liver injury in ethanol-fed rats



Alcoholism Clinical & Experimental Research 24(3): 332-335, March



Background: The role of free radicals in alcoholic liver injury remains uncertain. These experiments were conducted to measure radical formation in rats that were fed alcohol along with either fish oil or saturated fats, which cause different types of liver pathology. Methods: Liquid diets containing alcohol or isocaloric dextrose were administered to rats by intragastric infusion for 2 weeks. Radical intermediates detected by spin trapping were measured in bile. Results: In rats that were fed alcohol plus fish oil, biliary concentrations of trapped radicals, which most likely originated from lipids, were 6-fold higher than in controls that were fed fish oil plus dextrose. High rates of radical formation persisted 24 hr after alcohol withdrawal, when all alcohol had been metabolized. In contrast, diets containing alcohol and medium chain triglycerides did not stimulate lipid radical formation. Conclusions: High rates of lipid radical formation were observed only in rats that were fed alcohol in combination with a fish oil diet, and a persistent flux of radical formation continued after alcohol withdrawal. These radical phenomena precede serious liver pathology, which develops after longer periods of fish oil plus alcohol diets.

Accession: 011210929

Download citation: RISBibTeXText

PMID: 10776670

DOI: 10.1111/j.1530-0277.2000.tb04615.x

Download PDF Full Text: Pronounced hepatic free radical formation precedes pathological liver injury in ethanol-fed rats



Related references

Pronounced hepatic free radical formation precedes pathological liver injury in ethanol-fed rats. Toxicological Sciences 72(S-1): 55, March, 2003

Nf-kappab activation is associated with free radical generation and endotoxemia and precedes pathological liver injury in experimental alcoholic liver disease. Cytokine 16(1): 36-39, 2001

Reactive free radical generation in vivo in heart and liver of ethanol-fed rats: correlation with radical formation in vitro. Proceedings of the National Academy of Sciences of the United States of America 84(24): 9223-9227, 1987

Role of cytochrome P4502E1-dependent formation of hydroxyethyl free radical in the development of liver damage in rats intragastrically fed with ethanol. Hepatology 23(1): 155-163, 1996

Activities of hepatic enzymes related to ethanol oxidation and effects of ethanol on hepatic metabolites in rats with chronic liver injury. Enzyme 24(3): 142-151, 1979

Oxygen free radical production in rat liver Effect of different doses of ethanol on anoxia-reperfusion injury. Hepatology 30(4 PART 2): 553A, 1999

Oxygen free radical production in rat liver: dose-related effect of ethanol on reperfusion injury. Digestive Diseases and Sciences 46(5): 1057-1066, 2001

Antioxidant and free radical-scavenging enzymes in chronically ethanol-consuming rats: controversy over hepatic lipid peroxidation. Drug and Alcohol Dependence 22(1-2): 161-164, 1988

Medium-chain triglycerides inhibit free radical formation and TNF-a production in rats given enteral ethanol. American Journal of Physiology 278(3): 67-G476, 2000

Tempol, an intracelullar free radical scavenger, reduces liver injury in hepatic ischemia-reperfusion in the rat. Transplantation Proceedings 36(4): 849-853, 2004

Chemiluminescent measurement of increased free radical formation after ischemia/reperfusion. Mechanisms of free radical formation in the liver. Digestive Diseases and Sciences 40(5): 1045-1053, 1995

Dietary ginger impact on hepatic antioxidant enzyme homeostasis under ethanol induced free radical stress conditions in albino rats. 2007

Free radical formation precedes thromboxane in secondary ischemia. Surgical Forum (Chicago) 42: 542-545, 1991

MCI-186 (edaravone), a free radical scavenger, attenuates hepatic warm ischemia-reperfusion injury in rats. Transplant International 18(7): 844-853, 2005

Medium-chain triglycerides inhibit free radical formation and TNF-alpha production in rats given enteral ethanol. American Journal of Physiology. Gastrointestinal and Liver Physiology 278(3): G467-G476, 2000