Protein kinase Calpha is involved in the cell condensation during chondrogenesis in vitro

Lim, Y.B.; Kang, S.S.; Lee, Y.S.; Sonn, J.K.

Korean Journal of Biological Sciences 4(4): 361-366


ISSN/ISBN: 1226-5071
Accession: 011218218

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In order to investigate the role of protein kinase C (PKC) in chondrogenic differentiation, we examined the localization of PKC isoforms in a limb bud micromass culture system. PKCalpha is specifically localized in the regions which would become cartilage nodules, while PKClambda/iota and zeta display widespread distribution in the whole culture. Distribution of PKCalpha change along with promotion or inhibition of chondrogenesis by lysophosphatidylcholine or phorbol 12-myristate 13-acetate. On the other hand, localization of PKClambda/iota or zeta was not changed by the modulation of chondrogenesis. Peanut agglutinin binding protein which is associated with cell aggregation during chondrogenesis was present in the cell condensation regions and its expression in those regions was influenced by PKC activity. Expression of fibronectin and N-cadherin in the cell condensing area were also affected by modulation of PKC activity. These results suggest involvement of PKCalpha in the cell condensation, possibly through regulating expression of fibronectin and N-cadherin.