+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Purification and characterization of an antiviral ribosome-inactivating protein from Chenopodium album L

Purification and characterization of an antiviral ribosome-inactivating protein from Chenopodium album L

Agricultural Chemistry & Biotechnology 43(3): 125-130

An antiviral protein (CAP30) with ribosome-inactivating activity was purified from the leaves of Chenopodium album L. through ammonium sulfate precipitation and column chromatography using S-Sepharose, Blue-Sepharose, FPLC Suprose12 HR, and FPLC Mono-S. The molecular weight of CAP30 was estimated to be 30 kD. CAP30 was thermostable, maintaining its activity even after incubation at 70degreeC for 30 min, and was stable in the pH range of 6 to 9. In a cell-free in vitro translation system using rabbit reticulocyte lysate, protein synthesis was inhibited by the addition of CAP30 with an IC50 of 2.26 pM. The comparison of N-terminal amino acid sequences of this protein with the known ribosome-inactivating proteins (RIPs) revealed that it had some sequence homology with PAP-S and PAP-R from pokeweed (Phytolacca americana) and dodecandrin from P. dodecandra, but had no sequence homology with RIPs from other plants belonging to different orders. The mosaic symptoms on tobacco leaves caused by cucumber mosaic virus infection was completely inhibited by 100 ng/ml of the pure CAP30 protein.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 011226619

Download citation: RISBibTeXText

Related references

Antiviral activity of a type 1 ribosome-inactivating protein from Chenopodium album L. Agricultural Chemistry & Biotechnology 42(4): 161-165, 1999

Ribosome-inactivating activity and cDNA cloning of antiviral protein isoforms of Chenopodium album. Molecules and Cells 17(1): 73-80, 2004

Purification and characterization of four isoforms of Himalayan mistletoe ribosome-inactivating protein from Viscum album having unique sugar affinity. Archives of Biochemistry and Biophysics 423(2): 288-301, 2004

Molecular characterization of the recombinant A-chain of a type II ribosome-inactivating protein (RIP) from Viscum album coloratum and structural basis on its ribosome-inactivating activity and the sugar-binding properties of the B-chain. Journal of Biochemistry and Molecular Biology 39(5): 560-570, 2006

Purification, characterization and cloning of antiviral/ribosome inactivating protein from Amaranthus tricolor leaves. Phytochemistry 67(17): 1865-1873, 2006

Ribosome-inactivating proteins in edible plants and purification and characterization of a new ribosome-inactivating protein from Cucurbita moschata. Biochimica et Biophysica Acta 1760(5): 783-792, 2006

Purification and partial characterization of antiviral proteins from Chenopodium album L. leaves. Journal of Plant Physiology 156(5/6): 808-810, 2000

Isolation and characterization of two protein isoforms with antiviral activity from Chenopodium album L leaves. Journal of Plant Biochemistry and Biotechnology 12(2): 117-122, 2003

CDNA cloning and characterization of a ribosome inactivating protein of a hemi-parasitic plant (Viscum album L.) from North-Western Himalaya (India). Plant Science 168(3): 615-625, 2005

Purification and characterization of trichomaglin--a novel ribosome-inactivating protein with abortifacient activity. Biochemistry and Molecular Biology International 47(2): 185-193, 1999

Purification and characterization of a novel ribosome-inactivating protein from seeds of Trichosanthes kirilowii Maxim. Protein Expression and Purification 67(2): 120-125, 2009

Purification and characterization of a new ribosome inactivating protein from cinchonaglycoside C-treated tobacco leaves. Journal of Integrative Plant Biology 49(9): 1327-1333, 2007

Purification of a new ribosome-inactivating protein from the seeds of Cinnamomum porrectum and characterization of the RNA N-glycosidase activity of the toxic protein. Biological Chemistry 377(12): 825-831, 1996

Purification and characterization of abelesculin, a novel ribosome-inactivating protein from the mature seeds of Abelmoschus esculentus. Journal of Natural Medicines 61(2): 170-174, 2007

Purification and functional characterization of recombinant balsamin, a ribosome-inactivating protein from Momordica balsamina. International Journal of Biological Macromolecules 114: 226-234, 2018