EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Rapid identification of thermotolerant Campylobacter jejuni, Campylobacter coli, Campylobacter lari, and Campylobacter upsaliensis from various geographic locations by a GTPase-based PCR-reverse hybridization assay


Rapid identification of thermotolerant Campylobacter jejuni, Campylobacter coli, Campylobacter lari, and Campylobacter upsaliensis from various geographic locations by a GTPase-based PCR-reverse hybridization assay



Journal of Clinical Microbiology 37(6): 1790-1796



ISSN/ISBN: 0095-1137

PMID: 10325325

Recently, a gene from Campylobacter jejuni encoding a putative GTPase was identified. Based on two semiconserved GTP-binding sites encoded within this gene, PCR primers were selected that allow amplification of a 153-bp fragment from C. jejuni, C. coli, C. lari, and C. upsaliensis. Sequence analysis of these PCR products revealed consistent interspecies variation, which allowed the definition of species-specific probes for each of the four thermotolerant Campylobacter species. Multiple probes were used to develop a line probe assay (LiPA) that permits analysis of PCR products by a single reverse hybridization step. A total of 320 reference strains and clinical isolates from various geographic origins were tested by the GTP-based PCR-LiPA. The PCR-LiPA is highly specific in comparison with conventional identification methods, including biochemical and whole-cell protein analyses. In conclusion, a simple method has been developed for rapid and highly specific identification of thermotolerant Campylobacter species.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 011245913

Download citation: RISBibTeXText

Related references

Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. Journal of Medical Microbiology 56(Part 11): 1467-1473, 2007

Molecular discrimination between Campylobacter jejuni, Campylobacter coli, Campylobacter lari and Campylobacter upsaliensis by polymerase chain reaction based on a novel putative GTPase gene. Molecular and Cellular Probes 11(3): 177-185, 1997

Development of a multiplex PCR assay for identification of Campylobacter coil, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. Journal of Medical Microbiology 56(11): 1467-1473, 2007

Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid a gene lpxA. Journal of Clinical Microbiology 42(12): 5549-5557, 2004

Simultaneous Identification of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari with SmartCycler-Based Multiplex Quantitative Polymerase Chain Reaction. Foodborne Pathogens and Disease 14(7): 371-378, 2017

Differentiation of campylobacter jejuni campylobacter coli campylobacter lari and campylobacter fetus based on the parietal fatty acids profiles obtained by gas liquid chromatography mass spectrometry as well as on the hippurate hydrolysis. Mitteilungen aus dem Gebiete der Lebensmitteluntersuchung und Hygiene 83(2): 142-150, 1992

Pulsed-field gel electrophoretic discrimination of Campylobacter lari from two other thermophilic species of Campylobacter (Campylobacter coli and Campylobacter jejuni). Physico Chemical Biology 37(2): 63-67, 1993

Rapid detection and differentiation of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari in food, using multiplex real-time PCR. Journal of Food Protection 73(2): 241-250, 2010

Identification of Campylobacter jejuni, Campylobacter coli and Campylobacter lari by the nucleic acid amplification system NASBR. Journal of Applied Microbiology 77(6): 694-701, 1994

Identification of Campylobacter jejuni, Campylobacter coli and campylobacter lari by the nucleic acid amplification system NASBAR. Journal of Applied Bacteriology 77(6): 694-701, 1994

Rapid Detection of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari in Fresh Chicken Meat and By-Products in Bangkok, Thailand, Using Modified Multiplex PCR. Journal of Food Protection 78(7): 1363-1369, 2015

Nachweis von Campylobacter jejuni und Campylobacter coli in Kotproben gesunder Schlachtschweine in Abhängigkeit von der Jahreszeit - Mise en évidence de Campylobacter jejuni et Campylobacter coli dans des matières fécales de porcs sains aux abattoirs en fonction de la saison - Isolation of Campylobacter jejuni and Campylobacter coli from faeces samples of healthy slaughter pigs in relation to season of year. Zentralblatt für Veterinarmedizin. Reihe B. Infectious Diseases, Immunology, Food Hygiene, Veterinary Public Health 32(1): 40-45, 1985

Advantages of peptide nucleic acid oligonucleotides for sensitive site directed 16S rRNA fluorescence in situ hybridization (FISH) detection of Campylobacter jejuni, Campylobacter coli and Campylobacter lari. Journal of Microbiological Methods 62(2): 211-219, 2005

Development of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the detection and identification of Campylobacter jejuni, Campylobacter coli and Campylobacter fetus. Fems Immunology and Medical Microbiology 52(2): 260-266, 2008

Prevalence of Campylobacter jejuni, Campylobacter lari, and Campylobacter coli in different ecological guilds and taxa of migrating birds. Applied and Environmental Microbiology 68(12): 5911-5917, 2002