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Rapid screening method for residual veterinary drugs in meat and fish by HPLC



Rapid screening method for residual veterinary drugs in meat and fish by HPLC



Journal of the Food Hygienic Society of Japan 39(6): 383-390



A simple and rapid screening method using HPLC for the simultaneous determination of 13 veterinary drugs (oxytetracycline, quinoxaline-2-carboxylic acid, thiabendazole, 5-hydroxythiabendazole, albendazole-2-aminosulfone, sulfadimidine, flubendazole, zeranol, beta-trenbolone. sulfadimethoxine, sulfamonomethoxine, oxolinic aid, and enrofloxacin) in meat and fish has been developed. The drugs were extracted with 0.25% metaphosphoric acidmethanol-acetonitrile (6:2:2, v/v), and the extracts were cleaned up on an OASIS HLB (60 mg) cartridge. The HPLC separation was performed on a Wakosil-II-5C18 RS column (150 X 4.6 mm i.d.) with a gradient system of 0.025 mol/L phosphate buffer (pH 2.5)-acetonitrile as the mobile phase at a flow rate of 0.6 mL/min. The drugs were detected with ultraviolet (UV) and fluorescence detectors with time programming. The calibration graphs were rectilinear from 1 to 20 ng for each drug. The recoveries at levels of 0.02-0.1 mug/g were 75.1apprx90.5%.

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Accession: 011246735

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DOI: 10.3358/shokueishi.39.6_383


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