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Rapid solid-phase synthesis of DNA-binding pyrrole-imidazole polyamides



Rapid solid-phase synthesis of DNA-binding pyrrole-imidazole polyamides



Bioorganic & Medicinal Chemistry Letters 12(16): 2129-2132, 19 August



Pyrrole-imidazole polyamides can be synthesized to target predetermined sequences of DNA with nanomolar affinity and high specificity, and have been shown to modulate gene transcription both in vitro and in vivo. To make polyamides more readily available to biological laboratories, we have developed a rapid solid-phase synthesis based on azabenzotriazole (OAt) activation that decreases synthesis time 60% compared to standard benzotriazole (OBt) techniques, without loss of yield or purity.

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Accession: 011246836

Download citation: RISBibTeXText

PMID: 12127520

DOI: 10.1016/s0960-894x(02)00359-1


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