+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Reaction of reducing hydroxyl radical adducts of pyrimidine nucleotides with riboflavin and flavin adenine dinucleotide (FAD) via electron transfer: A pulse radiolysis study

Reaction of reducing hydroxyl radical adducts of pyrimidine nucleotides with riboflavin and flavin adenine dinucleotide (FAD) via electron transfer: A pulse radiolysis study

Biophysical Chemistry 85(1): 17-24

Using the techniques of pulse radiolysis with time-resolved spectrophotometric detection, it has been demonstrated that the interaction of reducing OH radical adducts of dCMP, TMP and UMP with riboflavin (RF) and flavin adenine dinucleotide (FAD) does proceed via an electron transfer reaction. From buildup kinetics of radical species, the rate constants of electron transfer from reducing OH adducts of pyrimidines to RF and FAD have been determined, respectively. It could be deduced that RF and FAD would reduce the probability of repair of the damaged DNA in the presence of enzymes and antioxidants, accordingly RF and FAD might have a radiosensitization effect on DNA damage.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 011249732

Download citation: RISBibTeXText

PMID: 10885395

DOI: 10.1016/s0301-4622(00)00131-9

Related references

Kinetic observation of rapid electron transfer between pyrimidine electron adducts and sensitizers of riboflavin, flavin adenine dinucleotide (FAD) and chloranil: a pulse radiolysis study. Radiation Physics and Chemistry 59(1): 61-66, 2000

Repair of dGMP hydroxyl radical adducts by verbascoside via electron transfer: A pulse radiolysis study. International Journal of Radiation Biology 69(4): 481-485, 1996

Sites of attack by hydroxyl free radical on nucleotides and dna a pulse radiolysis study. Biophysical Society Annual Meeting Abstracts 15: 178A, 1971

Nucleotides. XI. Some preliminary experiments on a projected synthesis of flavin-adenine dinucleotide. New methods for the preparation of riboflavin-4 :5 phosphate. Jour Chem Soc [london] (7): 2530-2535, 1952

Effects of riboflavin deficiency upon concentrations of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in Novikoff hepatoma in rats. Cancer Research 33(11): 3019-3023, 1973

Pharmacokinetic profile of flavin adenine dinucleotide, flavin mononucleotide and riboflavin following intravenous administration of riboflavin or its coenzymes in rats. Journal of Health Science 53(3): 332-338, 2007

Riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation. Clinical Chemistry 48(9): 1571-1577, 2002

Organic Phosphates. IX. An Effective Separation of Flavin Nucleotides and Adenine Nucleotide from Hydrolysates of Flavin Adenine Dinucleotide. Chemical & Pharmaceutical Bulletin 8(5): 436-440, 1960

Pulse radiolysis study of the reactivity of chloramphenicol, diazepam and clonazepam with the electron and the hydroxyl radical. Journal de Pharmacie de Belgique 48(6): 457-462, 1993

Initial characterization of the primary photochemistry of AppA, a blue-light-using flavin adenine dinucleotide-domain containing transcriptional antirepressor protein from Rhodobacter sphaeroides: a key role for reversible intramolecular proton transfer from the flavin adenine dinucleotide chromophore to a conserved tyrosine?. Photochemistry and Photobiology 78(3): 290-297, 2003

Reaction of hydroxyl radical with phenylpropanoid glycosides from Pedicularis species: a pulse radiolysis study. Science in China. Series C, Life Sciences 39(2): 154-158, 1996

Differences in proton-coupled electron-transfer reactions of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) between buffered and unbuffered aqueous solutions. Journal of Physical Chemistry. B 117(44): 13755-13766, 2014

Time-dependent yield of the hydrated electron and the hydroxyl radical in D2O: a picosecond pulse radiolysis study. Physical Chemistry Chemical Physics, 2018

Inhibiting intramolecular electron transfer in flavin adenine dinucleotide by host-guest interaction: a fluorescence study. Journal of Physical Chemistry. B 114(8): 2617-2626, 2010

A pulse radiolysis study of the reactions of the hydrated electron and hydroxyl radical with the oxalate ion in neutral aqueous solution. High Energy Chemistry 42(1): 1-6, 2008