Section 12
Chapter 11,252

Real-time quantitative PCR detection of genetically modified maximizer maize and roundup ready soybean in some representative foods

Vaïtilingom, M.; Pijnenburg, H.; Gendre, F.; Brignon, P.

Journal of Agricultural and Food Chemistry 47(12): 5261-5266


ISSN/ISBN: 0021-8561
PMID: 10606606
DOI: 10.1021/jf981208v
Accession: 011251091

A fast and quantitative method was developed to detect transgenic "Maximizer" maize "event 176" (Novartis) and "Roundup Ready" soybean (Monsanto) in food by real-time quantitative PCR. The use of the ABI Prism 7700 sequence detection system allowed the determination of the amplified product accumulation through a fluorogenic probe (TaqMan). Fluorescent dyes were chosen in such a way as to coamplify total and transgenic DNA in the same tube. Using real-time quantitative PCR, 2 pg of transgenic or total DNA per gram of starting sample was detected in 3 h after DNA extraction and the relative amounts of "Maximizer" maize and "Roundup Ready" soybean in some representative food products were quantified.

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