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Refined crystal structure of a superoxide dismutase from the hyperthermophilic archaeon Sulfolobus acidocaldarius at 2.2 A resolution

Knapp, S.; Kardinahl, S.; Hellgren, N.; Tibbelin, G.; Schäfer, G.; Ladenstein, R.

Journal of Molecular Biology 285(2): 689-702

1999


ISSN/ISBN: 0022-2836
PMID: 9878438
DOI: 10.1006/jmbi.1998.2344
Accession: 011262868

The extremely thermostable superoxide dismutase from the hyperthermophilic archaeon Sulfolobus acidocaldarius was crystallized and the three-dimensional structure was determined by X-ray diffraction methods. The enzyme crystallized in the monoclinic spacegroup C2 with the cell dimensions a = 168.1 ANG, b = 91.3 ANG, c = 85.7 ANG, beta = 91.4degree. The diffraction limit of these crystals was 2.2 ANG. The crystals were very stable in the X-ray beam and measured diffraction data of a single crystal had a completeness of 99.5% up to a resolution of 2.2 ANG. The crystal structure of S. acidocaldarius superoxide dismutase was solved by Patterson search methods using a dimer of Thermus thermophilus superoxide dismutase as a search model. The asymmetric unit accommodates three dimers. Two dimers form a tetramer by using only local symmetries; the third dimer forms a tetramer as well, however, by using the crystallographic 2-fold symmetry. The three-dimensional structure of the S. acidocaldarius dismutase has typical features of tetrameric dismutases. Secondary structure elements as well as residues important for the catalytic activity of the enzyme were found to be highly conserved. The model was refined at a resolution of 2.2 ANG and yielded a crystallographic R-value of 17.4% (Rfree = 22.3 %). A structural comparison of the two extremely stable tetrameric dismutases from S. acidocaldarius and Aquifex pyrophilus with the less stable enzyme from T. thermophilus and Mycoplasma tuberculosis revealed the structural determinants which are probably responsible for the high intrinsic stability of S. acidocaldarius dismutase. The most obvious factor which may give rise to the extraordinary thermal stability of S. acidocaldarius dismutase (melting temperature of about 125degreeC) is the increase in intersubunit ion pairs and hydrogen bonds and, more importantly, the significant reduction of solvent-accessible hydrophobic surfaces, as well as an increase in the percentage of buried hydrophobic residues.

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