+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Regeneration of sciatic nerves of adult rats induced by extracts from distal stumps of pre-degenerated peripheral nerves



Regeneration of sciatic nerves of adult rats induced by extracts from distal stumps of pre-degenerated peripheral nerves



Journal of Neuroscience Research 72(3): 417-424



Despite numerous experimental and clinical attempts to reconstruct injuries of peripheral nerves, the methods developed until now have not been sufficiently effective. We examined the influence of extracts (postmicrosomal fractions) obtained from non-pre-degenerated or 7-day-pre-degenerated distal segments of peripheral nerves on the regeneration of injured sciatic nerves of male adult rats. The extracts were introduced to the site of injury with autologous connective tissue chambers filled with fibrin. Reference groups were treated with brain-derived neurotrophic factor (BDNF) or fibrin only. We examined DiI-labeled motoneurons, toluidine blue-labeled myelinated fibers in the mid-part of the chambers, and AChE-positive nerve endings to assess the regeneration intensity. In addition, the length of fibers regrowing within the chambers was measured. We found that extracts obtained from distal stumps of 7-day-pre-degenerated peripheral nerves enhanced nerve regeneration as strongly as BDNF.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011263727

Download citation: RISBibTeXText

PMID: 12692908

DOI: 10.1002/jnr.10560


Related references

Neurotrophic activity of extracts from distal stumps of pre-degenerated peripheral rat nerves varies according to molecular mass spectrum. Neurological Research 30(8): 845-851, 2008

Metalloproteinases activity (MMP-2, MMP-9) in distal stumps of rat sciatic nerves. Wiadomosci Lekarskie 58(7-8): 411-414, 2005

Oxidized galectin-1 stimulates the migration of Schwann cells from both proximal and distal stumps of transected nerves and promotes axonal regeneration after peripheral nerve injury. Journal of Neuropathology and Experimental Neurology 62(2): 162-172, 2003

Notes on neurological semiology. XVII. I. Peripheral nerves. b) Spinal nerves. Sciatic popliteal nerves, external and internal. Concours Medical 84: 4305-4309, 1962

Fine structural and immunohistochemical identification of perineurial cells connecting proximal and distal stumps of transected peripheral nerves at early stages of regeneration in silicone tubes. Acta Neuropathologica. 88(2): 159-165, 1994

Tropism in nerve regeneration in vivo. Attraction of regenerating axons by diffusible factors derived from cells in distal nerve stumps of transected peripheral nerves. Brain Research 253(1-2): 1-12, 1982

Effects of Axotomy on the Cholinesterase and Carbonic Anhydrase Activities of Axons in the Proximal and Distal Stumps of Rabbit Sciatic Nerves. Plastic & Reconstructive Surgery 87(4): 726-738, 1991

Spatiotemporal expression of chondroitin 6 - sulfotransferase in regeneration of crush - injured sciatic nerves of adult rats. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 41 2, 2003

Effects of axotomy on the cholinesterase and carbonic anhydrase activities of axons in the proximal and distal stumps of rabbit sciatic nerves: a temporal study. Plastic and Reconstructive Surgery 87(4): 726, 1991

Pre-degenerated peripheral nerve extracts applied to the proximal stump of transected sciatic nerve enhance both regeneration and autotomic behavior in rats. Medical Science Monitor 8(10): Br414-Br420, 2002

Acetyl-L-carnitine prevents age-dependent structural alterations in rat peripheral nerves and promotes regeneration following sciatic nerve injury in young and senescent rats. Experimental Neurology 128(1): 103-114, 1994

Pharmacological studies on degeneration and regeneration of peripheral nerves. (1) Effects of methylcobalamin and cobamide on EMG patterns and loss of muscle weight in rats with crushed sciatic nerve. Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica 72(2): 259-268, 1976

Structural changes of peripheral nerves in the course of life morphometric investigations on sciatic and sural nerves. Zentralblatt fuer Allgemeine Pathologie und Pathologische Anatomie 136(6): 563-569, 1990

Structural changes in the peripheral nerves over a lifetime. Morphometric studies of the sciatic and sural nerves. Zentralblatt für Allgemeine Pathologie U. Pathologische Anatomie 136(6): 563-569, 1990

The regeneration of transected sciatic nerves of adult rats using chitosan nerve conduits seeded with bone marrow stromal cell-derived Schwann cells. Biomaterials 32(3): 787-796, 2011