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Selection of hydroxyproline-resistant cell lines from Solanum tuberosum L. Callus II. Plant regeneration and frost tolerance of regenerated plants



Selection of hydroxyproline-resistant cell lines from Solanum tuberosum L. Callus II. Plant regeneration and frost tolerance of regenerated plants



Acta Biotechnologica 18(4): 361-366



The calluses of two hydroxyproline-resistant lines (D20-1 and D30-1) of Solanum tuberosum L. were transferred to a solidified MS medium containing 1.0 mg/l IAA 2.0 mg/l zeatin, 40.0 mg/l adenine sulphate, 1 g/l casein hydrolysate, 20 g/1 sucrose and 10 g/l agar for plant regeneration. The shoot regeneration was only achieved from the callus of line D20-1. Regenerated shoots exhibited morphological variability. The degrees of frost tolerance were higher in the leaves of the regenerated plants compared with the leaves of the non-selected control plants, but lower than that of the callus from which they were regenerated.

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Accession: 011335510

Download citation: RISBibTeXText

DOI: 10.1002/abio.370180412



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