Sequence analysis of the gene encoding ompL1 and flaB2 from Leptospira interrogans serovar lai and Leptospira interrogans serovar australis
Wang Min; Dai Baomin
Zhonghua Weishengwuxue He Mianyixue Zazhi 22(3): 250-252
Objective To construct recombinant plasmids from Leptospira interrogans serovar lai strain 017 and Leptospira interrogans serovar australis strain 607 consisted of an outer membrane protein gene (ompL1) and a flagellin B gene (flaB2). The ompL1 and flaB2 gene were analyzed by DNA sequencing. Methods The DNA fragments of ompL1 and flaB2 were respectively amplified by PCR, then about cloned into the down stream of a T7 promoter of vector pcDNA3. 1/Myc-His(+), recombinant plasmids of the antigen gene were constructed, and analyzed by DNA sequencing. Results Analysis of the DNA sequence of ompL1 from L. interrogans lai strain 017 and L. interrogans australis strain 607 showed that the similarity of nucleotide sequence was 98.85% (949/960), variation was 1.15% (11/960); About flaB2, the similarity was 96.94% (823/849), variation was 3.06% (26/849), the sequences of each were highly conserved. Conclusion sequences analysis of ompL1 and flaB2 from Leptospira interrogans serovar lai strain 017 respectively revealed a high level of identity with that from Leptospira interrogans serovar australis strain 607.