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Sequencing and sequence analysis of S1 spike protein gene from avian infectious bronchitis virus proventriculus strain isolated in Qingdao (SD/97/02)



Sequencing and sequence analysis of S1 spike protein gene from avian infectious bronchitis virus proventriculus strain isolated in Qingdao (SD/97/02)



Virologica Sinica 16(4): 377-381



A pair of primers were designed and synthesized according to the reported S1 spike protein gene sequence of IBV. The viral RNA of SD/97/02 strain was amplified by reverse transcription-polymerase chain reaction (RT-PCR). The amplified product was analyzed by agarose gel electrophoresis, all appeared a fragment of 1657 bp as expected. The RT-PCR product was cloned into pMD18-T vector, and then the recombinant plasmid was sequenced. The result suggested that it was the S1 spike gene of IBV.

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