Section 12
Chapter 11,356

Short-period mutations of per affect a double-time-dependent step in the Drosophila circadian clock

Rothenfluh, A.; Abodeely, M.; Young, M.W.

Current Biology Cb 10(21): 1399-1402


ISSN/ISBN: 0960-9822
PMID: 11084344
DOI: 10.1016/s0960-9822(00)00786-7
Accession: 011355556

Download citation:  

Circadian (24 hour) PERIOD (PER) protein oscillation is dependent on the double-time (dbt) gene, a casein kinase Ivarepsilon homolog [1-3]. Without dbt activity, hypophosphorylated PER proteins over-accumulate, indicating that dbt is required for PER phosphorylation and turnover [3,4]. There is evidence of a similar role for casein kinase Ivarepsilon in the mammalian circadian clock [5,6]. We have isolated a new dbt allele, dbt(ar), which causes arrhythmic locomotor activity in homozygous viable adults, as well as molecular arrhythmicity, with constitutively high levels of PER proteins, and low levels of TIMELESS (TIM) proteins. Short-period mutations of per, but not of tim, restore rhythmicity to dbt(ar) flies. This suppression is accompanied by a restoration of PER protein oscillations. Our results suggest that short-period per mutations, and mutations of dbt, affect the same molecular step that controls nuclear PER turnover. We conclude that, in wild-type flies, the previously defined PER'short domain' [7,8] may regulate the activity of DBT on PER.

PDF emailed within 0-6 h: $19.90