+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Significant up-regulation of nestin protein in the neostriatum of MPTP-treated mice. Are the striatal astrocytes regionally activated after systemic MPTP administration?



Significant up-regulation of nestin protein in the neostriatum of MPTP-treated mice. Are the striatal astrocytes regionally activated after systemic MPTP administration?



Brain Research 925(1): 9-17, 18 January



We are interested in the possible role of central glial cells in pathogenesis of Parkinson's disease of mammals. Parkinsonism model was induced by systemic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration, and the reactive glial cells were examined by immunocytochemical visualization of nestin protein in the brains and spinal cords of C57 mice. Abundant nestin-like immunoreactivity was predominately found in the caudate putamen of MPTP-treated mice and about 481-fold of nestin-like immunoreactive cells increased compared with that of control animals, indicating that significant up-regulation of nestin protein occurred in these regions. Majority of nestin-like immunoreactive cells characterized with astrocytic profiles of multiple, radical and hypotrophic processes, and showed a distribution and dynamic patterns similar to that of glial fibrillary acid protein (GFAP)-immunoreactive cells in the caudate putamen. Double immunofluorescence confirmed that 100% of nestin-like immunoreactive cells exhibited GFAP-immunoreactivity while nestin/GFAP double-labeled cells constituted about 84% of total GFAP-immunoreactive cells in the caudate putamen, indicating these nestin-like immunoreactive cells belong to a reactive population of the astrocytes. On the other hand, no obvious changes of nestin- or GFAP-like immunoreactivities were detected in the globus pallidus, the substantia nigra and the ventral tegmental area after MPTP-treatment. The results have provided morphological evidence for the regional activation of astrocytic glial cells following systemic MPTP administration, suggesting that a large population of reactive striatal astrocytes might play an important role in initial pathogenesis or acute stage of Parkinson's disease in mammals.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011359445

Download citation: RISBibTeXText

PMID: 11755896

DOI: 10.1016/s0006-8993(01)03253-x


Related references

Protection or rescue of dopamine neurons in mptp treated mice is dependent upon the mptp administration protocol. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 485 12, 2002

Sustained depletion of cortical and hippocampal serotonin and norepinephrine but not striatal dopamine by 1-methyl-4-(2'-aminophenyl)-1,2,3,6-tetrahydropyridine (2'-NH-2-MPTP): A comparative study with 2'-CH-3-MPTP and MPTP. Journal of Neurochemistry 60(3): 1167-1170, 1993

Regulation of striatal preproenkephalin mRNA levels in MPTP-lesioned mice treated with estradiol. Journal of Neuroscience Research 80(1): 138-144, 2005

Transient reduction of tritiated spiroperidol binding to striatal tissue following chronic administration of prolylleucylglycinamide plg in mptp treated mice. Federation Proceedings 46(3): 391, 1987

Persistent depletion of striatal dopamine and cortical norepinephrine in mice by 1-methyl-4-phenyl-1,2,3,6-tetrahydro-3-pyridinol (MPTP-3-OL), an analog of MPTP. Life Sciences 50(8): 573-582, 1992

Novel sites of action for deprenyl in MPTP-parkinsonism: metabolite-mediated protection against striatal neurotoxicity and suppression of MPTP-induced increase of dopamine turnover in C57BL mice. Progress in Brain Research 106: 155-171, 1995

Increased plasma levels of TNF-alpha but not of IL1-beta in MPTP-treated monkeys one year after the MPTP administration. Parkinsonism and Related Disorders 11(7): 435-439, 2005

Tissue concentrations of MPTP and MPP+ after administration of lethal and sublethal doses of MPTP to mice. Toxicology Letters 54(2-3): 253-262, 1990

Tissue concentrations of mptp and mpp positive after administration of lethal and sublethal doses of mptp to mice. Toxicology Letters 54(2-3): 253-262, 1990

Tissue concentrations of MPTP and MPP+ in relation to catecholamine depletion after the oral or subcutaneous administration of MPTP to mice. Life Sciences 45(22): 2077-2083, 1989

Tissue concentrations of mptp and mpp positive in relation to catecholamine depletion after the oral or subcutaneous administration of mptp to mice. Life Sciences 45(22): 2077-2084, 1989

Preferential Heme Oxygenase-1 Activation in Striatal Astrocytes Antagonizes Dopaminergic Neuron Degeneration in MPTP-Intoxicated Mice. Molecular Neurobiology 53(8): 5056-5065, 2016

Decreased striatal manganese content in MPTP treated mice. Proceedings of the Western Pharmacology Society 34: 497-500, 1991

Increase of striatal dopamine turnover by stress in MPTP-treated mice. Clinical Neuropharmacology 11(4): 360-368, 1988

Postsynaptic supersensitivity in striatal D-1 dopamine receptors in MPTP-treated mice. Society for Neuroscience Abstracts 18(1-2): 1577, 1992