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Site-directed insertion and insertion-deletion mutations in the Escherichia coli chromosome simplified



Site-directed insertion and insertion-deletion mutations in the Escherichia coli chromosome simplified



Genetic Analysis Biomolecular Engineering 15(6): 239-244



A procedure to produce an exact chromosomal replica of an insertion or insertion-deletion mutation produced in vitro in a plasmid with a ColE 1 origin of replication is presented. This procedure uses a previously described property of recD mutations (Biek DP, Cohen SN. J. Bacteriol. 1986; 167: 594-603) and is limited by (1) the compatibility of the new mutation with recD; and (2) the presence of some Escherichia coli DNA flanking the mutation.

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Accession: 011365739

Download citation: RISBibTeXText

PMID: 10609761

DOI: 10.1016/s1050-3862(99)00031-5


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