+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Spatial pattern of constitutive and heat shock-induced expression of the small heat shock protein gene family, Hsp30, in Xenopus laevis tailbud embryos



Spatial pattern of constitutive and heat shock-induced expression of the small heat shock protein gene family, Hsp30, in Xenopus laevis tailbud embryos



Developmental Genetics 25(4): 365-374



We employed whole-mount in situ hybridization and immunohistochemistry to study the spatial pattern of hsp30 gene expression in normal and heatshocked embryos during Xenopus laevis development. Our findings revealed that hsp30 mRNA accumulation was present constitutively only in the cement gland of early and midtailbud embryos, while hsp30 protein was detected until at least the early tadpole stage. Heat shock-induced accumulation of hsp30 mRNA and protein was first observed in early and midtailbud embryos with preferential enrichment in the cement gland, somitic region, lens placode, and proctodeum. In contrast, cytoskeletal actin mRNA displayed a more generalized pattern of accumulation which did not change following heat shock. In heat shocked midtailbud embryos the enrichment of hsp30 mRNA in lens placode and somitic region was first detectable after 15 min of a 33 degrees C heatshock. The lowest temperature capable of inducing this pattern was 30 degrees C. Placement of embryos at 22 degrees C following a 1-h 33 degrees C heat shock resulted in decreased hsp30 mRNA in all regions with time, although enhanced hsp30 mRNA accumulation still persisted in the cement gland after 11 h compared to control. In late tailbud embryos the basic midtailbud pattern of hsp30 mRNA accumulation was enhanced with additional localization to the spinal cord as well as enrichment across the embryo surface. These studies demonstrate that hsp30 gene expression can be detected constitutively in the cement gland of tailbud embryos and that heat shock results in a preferential accumulation of hsp30 mRNA and protein in certain tissues.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011381849

Download citation: RISBibTeXText

PMID: 10570468

DOI: 10.1002/(sici)1520-6408(1999)25:4<365::aid-dvg10>3.0.co;2-2


Related references

Identification of members of the HSP30 small heat shock protein family and characterization of their developmental regulation in heat-shocked Xenopus laevis embryos. Developmental Genetics 17(4): 331-339, 1995

Comparison of the effect of heat shock factor inhibitor, KNK437, on heat shock- and chemical stress-induced hsp30 gene expression in Xenopus laevis A6 cells. Comparative Biochemistry and Physiology. Part A, Molecular and Integrative Physiology 151(2): 253-261, 2008

Examination of cadmium-induced expression of the small heat shock protein gene, hsp30, in Xenopus laevis A6 kidney epithelial cells. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology 152(1): 91-99, 2008

Examination of KNK437- and quercetin-mediated inhibition of heat shock-induced heat shock protein gene expression in Xenopus laevis cultured cells. Comparative Biochemistry and Physiology. Part A, Molecular and Integrative Physiology 148(3): 521-530, 2007

Analysis of the expression and function of the small heat shock protein gene, hsp27, in Xenopus laevis embryos. Comparative Biochemistry and Physiology. Part A, Molecular and Integrative Physiology 147(1): 112-121, 2007

Constitutive and stress-inducible expression of the endoplasmic reticulum heat shock protein 70 gene family member, immunoglobulin-binding protein (BiP), during Xenopus laevis early development. Developmental Genetics 25(1): 31-39, 1999

Mutation or deletion of the C-terminal tail affects the function and structure of Xenopus laevis small heat shock protein, hsp30. Comparative Biochemistry & Physiology Part B Biochemistry & Molecular Biology 133B(1): 95-103, 2002

Heat-shock-induced assembly of Hsp30 family members into high molecular weight aggregates in Xenopus laevis cultured cells. Comparative Biochemistry & Physiology B. 119(2): 381-389,., 1998

Celastrol can inhibit proteasome activity and upregulate the expression of heat shock protein genes, hsp30 and hsp70, in Xenopus laevis A6 cells. Comparative Biochemistry and Physiology. Part A, Molecular and Integrative Physiology 156(2): 285-293, 2010

Differential heat shock tolerance of animal and vegetal halves of xenopus laevis embryos correlates with distinct patterns of heat shock protein synthesis. Journal of Cell Biology 99(4 PART 2): 453A, 1984

Examination of the expression of the heat shock protein gene, hsp110, in Xenopus laevis cultured cells and embryos. Comparative Biochemistry and Physiology. Part A, Molecular and Integrative Physiology 145(2): 225-234, 2006

Heat shock gene expression in Xenopus laevis A6 cells in response to heat shock and sodium arsenite treatments. Biochemistry and Cell Biology 66(8): 862-870, 1988

Comparison of regulatory and structural regions of the Xenopus laevis small heat-shock protein-encoding gene family. Gene 110(2): 159-166, 1992

Effect of herbimycin A on hsp30 and hsp70 heat shock protein gene expression in Xenopus cultured cells. Biochemistry and Cell Biology 75(6): 777-782, 1997

On the model of feedback regulation of heat shock gene expression by heat shock proteins Demonstration of heat shock protein 70-containing complexes of unactivated heat shock transcription factor 1. Protein Engineering 8(SUPPL ): 35, 1995