Species identification of smoked and gravad fish products by sodium dodecylsulphate polyacrylamide gel electrophoresis, urea isoelectric focusing and native isoelectric focusing: A collaborative study

Mackie, I.; Craig, A.; Etienne, M.; Jerome, M.; Fleurence, J.; Jessen, F.; Smelt, A.; Kruijt, A.; Yman, I.M.; Ferm, M.; Martinez, I.; Perez Martin, R.; Pineiro, C.; Rehbein, H.; Kuendiger, R.

Food Chemistry 71(1): 1-7


ISSN/ISBN: 0308-8146
DOI: 10.1016/s0308-8146(00)00147-3
Accession: 011383796

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A collaborative study on the use of sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), urea-isoelectric focusing (urea-IEF) and native isoelectric focusing for the identification of species of smoked salmonids, gravad salmonids and smoked eels was carried out by eight laboratories. With SDS-PAGE, minor changes took place in the profiles of the processed salmonid species making it impossible or very difficult to identify closely related species. With urea-IEF, there were fewer changes in the profiles due to processing and the system generally had greater species-discriminating power for the processed salmonids than SDS-PAGE. The profiles of the eel species as obtained on SDS-PAGE or urea-IEF were not affected by smoking. Urea-IEF had greater species-discriminating power than SDS-PAGE for the eel species. Native IEF was useful in providing supplementary identification on species difficult to identify by SDS-PAGE or by urea-IEF in the case of cold smoked products.