Studies of transgenic tobacco plants expressing beta-1,3-glucanase and chitinase genes and their potential for fungal resistance
Lan, H.Y.; Tian, Y.C.; Wang, C.H.; Liu, G.Z.; Zhang, L.H.; Wang, L.L.; Chen, Z.H.
Yi Chuan Xue Bao 27(1): 70-77
ISSN/ISBN: 0379-4172 PMID: 10883543 Accession: 011412837
Plant bivalent expression vector pBLGC for basic tobacco beta-1, 3-glucanase and bean chitinase genes were constructed, and the tobacco tissue was transformed. Transgenic tobacco plants were carried out for analysis by PCR, PCR-Southern blot, Dot blot, Western blot, etc., and the results showed that some transgenic plants gave strong positive signal, it suggested that foreign genes have been integreted into tobacco genome, and expressed correctly. Fungal challenge with Alternaria alternata showed that transgenic plants were much more resistant to this pathogenic fungus.