Home
  >  
Section 12
  >  
Chapter 11,427

Superoxide electrode based on covalently immobilized cytochrome c: Modelling studies

Tammeveski, K.; Tenno, T.T.; Mashirin, A.A.; Hillhouse, E.W.; Manning, P.; Mcneil, C.J.

Free Radical Biology and Medicine 25(8): 973-978

1998

ISSN/ISBN: 0891-5849
PMID: 9840743
DOI: 10.1016/s0891-5849(98)00182-8
Accession: 011426843
Download citation:  
Text
  |  
BibTeX
  |  
RIS
We have recently described an optimized electrode for the detection of enzymatic and cellular superoxide (O2.-) production based on cytochrome c immobilized covalently at a surface-modified gold electrode and applied this system to the study of free radical production by activated human glioblastoma cells. In this paper we report the development of a mathematical model for the O2.- electrode responding to enzymically produced O2.- which should enable the determination of absolute concentrations of O2.- in biological systems when this electrode is employed for direct, real-time monitoring of free radical release and interactions.

PDF emailed within 0-6 h: $19.90




Related References

Rahman, M.A.; Kothalam, A.; Choe, E.S.; Won, M.-S.; Shim, Y.-B. 2012: Stability and sensitivity enhanced electrochemical in vivo superoxide microbiosensor based on covalently co-immobilized lipid and cytochrome c Analytical Chemistry 84(15): 6654-6660
Aminur Rahman, M.; Kothalam, A.; Eun Sang, C.H.O.E.; Won, M.S.; Shim, Y.B. 2012: Stability and Sensitivity Enhanced Electrochemical In Vivo Superoxide Microbiosensor Based on Covalently Co-immobilized Lipid and Cytochrome c Analytical Chemistry 84(15): 6754-6760
Zhou, Y.; Zhi, J.; Zou, Y.; Zhang, W.; Lee, S.-T. 2008: Direct electrochemistry and electrocatalytic activity of cytochrome c covalently immobilized on a boron-doped nanocrystalline diamond electrode Analytical Chemistry 80(11): 4141-4146
Darain, F.; Park, J.-S.; Akutsu, H.; Shim, Y.-B. 2007: Superoxide radical sensing using a cytochrome c3 immobilized conducting polymer electrode Biosensors and Bioelectronics 23(2): 161-167
Di, J.; Bi, S.; Zhang, M. 2004: Third-generation superoxide anion sensor based on superoxide dismutase directly immobilized by sol-gel thin film on gold electrode Biosensors and Bioelectronics 19(11): 1479-1486
Lagercrantz, C.; Larsson, T. 1983: Comparative studies of the binding of some ligands to human serum albumin non-covalently attached to immobilized Cibacron Blue, or covalently immobilized on Sepharose, by column affinity chromatography Biochemical Journal 213(2): 387-390
Chu, X.; Duan, D.; Shen, G.; Yu, R. 2007: Amperometric glucose biosensor based on electrodeposition of platinum nanoparticles onto covalently immobilized carbon nanotube electrode Talanta 71(5): 2040-2047
Tiwari, A.; Aryal, S.; Pilla, S.; Gong, S. 2009: An amperometric urea biosensor based on covalently immobilized urease on an electrode made of hyperbranched polyester functionalized gold nanoparticles Talanta 78(4-5): 1401-1407
Beissenhirtz, M.K.; Scheller, F.W.; Lisdat, F. 2004: A superoxide sensor based on a multilayer cytochrome c electrode Analytical Chemistry 76(16): 4665-4671
Thenmozhi, K.; Sriman Narayanan, S. 2007: Amperometric hydrogen peroxide sensor based on a sol-gel-derived ceramic carbon composite electrode with toluidine blue covalently immobilized using 3-aminopropyltrimethoxysilane Analytical and Bioanalytical Chemistry 387(3): 1075-1082
Ge, B.; Lisdat, F. 2002: Superoxide sensor based on cytochrome c immobilized on mixed-thiol SAM with a new calibration method Analytica Chimica Acta 454(1): 53-64
Ge, B.; Lisdat, F. 2002: Superoxide sensor based on cytochrome c immobilized on mixed-thiol SAM with a new calibration method Analytica Chimica Acta 454(1): 53-64
Guo, Z.; Chen, J.; Liu, H.; Zhang, W. 2005: Electrochemical determination of superoxide based on cytochrome c immobilized on DDAB-modified powder microelectrode Analytical Letters 38(13): 2033-2043
Nohwal, B.; Chaudhary, R.; Kumar, P.; Pundir, C.S. 2020: Fabrication and application of an amperometric lysine biosensor based on covalently immobilized lysine oxidase nanoparticles onto Au electrode International Journal of Biological Macromolecules 146: 907-915
Matsuda, N.; Santos, J.H.; Takatsu, A.; Kato, K. 2003: Spectroelectrochemical studies on surface immobilized cytochrome c on ITO electrode by slab optical waveguide spectroscopy Thin Solid Films 438-39: 403-406
Shipovskov, S.; Ferapontova, E.E.; Gazaryan, I.; Ruzgas, T. 2004: Recombinant horseradish peroxidase - and cytochrome c-based two-electrode system for detection of superoxide radicals Bioelectrochemistry 63(1-2): 277-280
Colosimo, A.; Brunori, M.; Antonini, E. 1976: Electron transfer between soluble and immobilized mammalian cytochrome c. Equilibrium and kinetic studies on immobilized cytochrome c Biochemical Journal 153(3): 657-661
Suzuki, T.; Yamamoto, K.; Tanaka, Y.; Daido, T.; Akaike, T. 1989: Design of bioelectronic device using cytochrome c mediation of electron transfer by cytochrome c immobilized on electrode Membrane 14(5): 319-328
Bogdanovskaya, V.A.; Tarasevich, M.R.; German Eh, D.; Gavrilova, E.F. 1989: Mécanisme lors de la transformation rédox du cytochrome-c immobilisé sur une électrode de pyrographite - Mechanism of the redox transformations of cytochrome-c immobilized of a pyrographite electrode Elektrohimia 25(7): 964-969
Collinson, M.; Bowden, E.F.; Tarlov, M.J. 1992: Voltammetry of covalently immobilized cytochrome c on self-assembled monolayer electrodes Langmuir 8(5): 1247-1250