Synthesis and biological effect of an anorectic peptide CART (cocaine- and amphetamine-regulated transcript) (human55-102) analogue
Synthesis and biological effect of an anorectic peptide CART (cocaine- and amphetamine-regulated transcript) (human55-102) analogue
Abiko, T.; Fujimura, R.
Protein and Peptide Letters 8(2): 147-151
2001
(Phe(4F)97)CART55-102 was synthesized by a solid-phase method. Hydrogen fluoride was used to deprotect and cleave the peptide from the resin except for Acm groups and a Met(0) residue. The product was treated with dithiothreitol to remove a sulfoxide group of Met(0) residue and then treated with Hg(OAc)2 in 50% aqueous AcOH to remove Acm groups. The reduced peptide was subjected to oxidative folding to form disulfide bridges. The crude product was purified by PR-HPLC.