+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Synthesis of novel heterobranched beta-cyclodextrins from 4(2)-O-beta-D-galactosyl-maltose and beta-cyclodextrin by the reverse action of pullulanase, and isolation and characterization of the products

Synthesis of novel heterobranched beta-cyclodextrins from 4(2)-O-beta-D-galactosyl-maltose and beta-cyclodextrin by the reverse action of pullulanase, and isolation and characterization of the products

Bioscience Biotechnology and Biochemistry 64(6): 1223-1229

From the mixture of 4(2)-O-beta-D-galactosyl-maltose (Gal-G2) and beta-cyclodextrin (betaCD), novel heterobranched betaCDs, (Gal-G2)-betaCD and (Gal-G2)2-betaCDs, were synthesized by the reverse action of debranching enzyme. The optimum conditions for the production of (Gal-G2)2-betaCDs were examined. A mixture of (Gal-G2)2-betaCDs was produced in about 4% yield when Aerobacter aerogenes pullulanase (64 units per 1 g of Gal-G2) was incubated with 1.6 M Gal-G2 and 0.16 M betaCD at 50 degrees C for 4 days. The reaction products, (Gal-G2)2-betaCDs, were separated into three peaks by HPLC analysis on a Hypercarb S column. Their structures were analyzed by fast atom bombardment mass spectroscopy and NMR spectroscopies, and confirmed by comparison of their hydrolyzates by beta-galactosidase with the authentic (G2)2 -betaCDs. The structures of (Gal-G2)-betaCD and three components of (Gal-G2)2-betaCDs were identified as 6-O-(GalG2)-betaCD, 6(1),6(2)-, 6(1),6(3)- and 6(1),6(4)-di-O-(Gal-G2)2-betaCD, respectively.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 011444479

Download citation: RISBibTeXText

PMID: 10923794

DOI: 10.1271/bbb.64.1223

Related references

Synthesis of novel fungicidal thiobarbituric acid derivatives. Egyptian Journal of Chemistry 43(4): 333-340, 2000

Synthesis of nonreducing-sugar subunit analogs of bacterial lipid A carrying the acyloxyacyl group of different carbon chain length. Agricultural and Biological Chemistry 54: 51-8, 1990

Synthesis of novel heteroaromatics structurally related to ellipticine alkaloids via thermolysis of pyridannulated enyne-carbodiimides. Journal of Organic Chemistry 67(15): 5412-5415, 2002

Synthesis of novel fullerene amino acids and their multifullerene peptides. Tetrahedron Letters 45(38): 7137-7140, 2004

Mucin synthesis 3 udp n acetyl d gluco pyranosylamine o beta d galacto pyranosyl 1 3 o n acetyl beta d glucopyradosylaminyl 1 6 n acetyl n o nitrophenyl or benzyl d galacto pyranose n acetyl d gluco pyranhosamine to d galacto pyranose beta 3 n acetyl d glucosaminyl transferase an enzyme in porcine gastric mucosa involved in the elongation of mucin type oligo saccharides. Canadian Journal of Biochemistry and Cell Biology 61(12): 1322-1333, 1983

Synthesis and properties of adenylyl 3' 5' uridine analogs containing 2' halo 2' deoxy adenosines effects of 2' substituents on oligo nucleotide conformation. Biochemistry 21(23): 5870-5877, 1982

Isolation and characterization of desulfovibrio senezii sp. nov., A halotolerant sulfate reducer from a solar saltern and phylogenetic confirmation of desulfovibrio fructosovorans as a new species. Archives of Microbiology 170(4): 313-317, 1998

Isolation and characterization of developmental variants in fruiting using a homokaryotic fruiting strain of Coprinus cinereus. Mycoscience 40(3): 227-233, 1999

Coordination dynamics of the horse-rider system. Journal of Motor Behavior 37(6): 418-424, 2005

Acceptor specificity and tissue distribution of three human alpha-3-fucosyltransferases. European Journal of Biochemistry 191(1): 169-176, 1990

Coordination dynamics of abdomen and chest during infant breathing A comparison of full-term and preterm infants at 38 weeks postconceptional age. Ecological Psychology. 11(3): 209-232, 1999

Acceptor specificity of cyclodextrin glucanotransferase from an alkalophilic Bacillus species and synthesis of glucosyl rhamnose. Bioscience Biotechnology and Biochemistry 60(7): 1176-1178, 1996

Preparation of DNA-carrying affinity latex and purification of transcription factors with the latex. Journal of Biomaterials Science Polymer Edition 5(4): 293-302, 1994

Studies of cyclodextrin inclusion complexes ii. molecular modelling and proton nmr evidence for the salbutamol beta cyclodextrin complex. International Journal of Pharmaceutics 63(3): 267-274, 1990

Complete sequences of the ribosome recognition sites in vesicular stomatitis virus messenger rna recognition by the 40s and 80s complexes. Cell 14(2): 345-354, 1978