+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Synthesis of structured triacylglycerols containing caproic acid by lipase-catalyzed acidolysis: Optimization by response surface methodology



Synthesis of structured triacylglycerols containing caproic acid by lipase-catalyzed acidolysis: Optimization by response surface methodology



Journal of Agricultural & Food Chemistry 49(12): 5771-5777



Production in a batch reactor with a solvent-free system of structured triacylglycerols containing short-chain fatty acids by Lipozyme RM IM-catalyzed acidolysis between rapeseed oil and caproic acid was optimized using response surface methodology (RSM). Reaction time (t(r)), substrate ratio (S(r)), enzyme load (E(l), based on substrate), water content (W(c), based on enzyme), and reaction temperature (T(e)), the five most important parameters for the reaction, were chosen for the optimization. The range of each parameter was selected as follows: t(r) = 5-17 h; E(l) = 6-14 wt %; T(e) = 45-65 degrees C; S(r) = 2-6 mol/mol; and W(c) = 2-12 wt %. The biocatalyst was Lipozyme RM IM, in which Rhizomucor miehei lipase is immobilized on a resin. The incorporation of caproic acid into rapeseed oil was the main monitoring response. In addition, the contents of mono-incorporated structured triacylglycerols and di-incorporated structured triacylglycerols were also evaluated. The optimal reaction conditions for the incorporation of caproic acid and the content of di-incorporated structured triacylglycerols were as follows: t(r) = 17 h; S(r) = 5; E(l) = 14 wt %; W(c) = 10 wt %; T(e) = 65 degrees C. At these conditions, products with 55 mol % incorporation of caproic acid and 55 mol % di-incorporated structured triacylglycerols were obtained.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011445019

Download citation: RISBibTeXText

PMID: 11743762

DOI: 10.1021/jf0103020


Related references

Production of structured phospholipids by lipase-catalyzed acidolysis: Optimization using response surface methodology. Enzyme & Microbial Technology 31(4): 523-532, 2 September, 2002

Modeling of lipase-catalyzed acidolysis of sesame oil and caprylic Acid by response surface methodology: optimization of reaction conditions by considering both acyl incorporation and migration. Journal of Agricultural and Food Chemistry 53(20): 8033-8037, 2005

Synthesis of structured triacylglycerols by lipase-catalyzed acidolysis in a packed bed bioreactor. Journal of agricultural and food chemistry 48(1): 3-10, 2000

Enrichment of hazelnut oil with long-chain n-3 PUFA by lipase-catalyzed acidolysis: optimization by response surface methodology. Journal of the American Oil Chemists' Society 82(1): 27-32, 2005

Lipase-catalyzed production of medium-chain triacylglycerols from palm kernel oil distillate: Optimization using response surface methodology. European Journal of Lipid Science and Technology 109(2): 107-119, 2007

Modeling and optimization of lipase-catalyzed synthesis of phytosteryl esters of oleic acid by response surface methodology. Food chemistry02(1): 336-342, 2007

Lipase-catalyzed acidolysis of algal oils with capric acid: optimization of reaction conditions using response surface methodolgy. Journal of Food Lipids 11(2): 147-163, 2004

Optimization of lipase-catalyzed synthesis of caffeic acid phenethyl ester in ionic liquids by response surface methodology. Bioprocess and Biosystems Engineering 36(6): 799-807, 2013

Quantitation of acyl migration during lipase-catalyzed acidolysis, and of the regioisomers of structured triacylglycerols formed. Journal of the American Oil Chemists' Society 78(9): 959-964, 2001

Lipase-catalyzed synthesis of isoamyl butyrate: Optimization by response surface methodology. Journal of the American Oil Chemists' Society 76(12): 1483-1488, 1999

Optimization of lipase-catalyzed synthesis of ginsenoside Rb1 esters using response surface methodology. Journal of Agricultural and Food Chemistry 56(22): 10988-10993, 2008

Optimization of lipase-catalyzed synthesis of diglycerol monooleate by response surface methodology. Biomass and Bioenergy 61: 179-186, 2014

Optimization of lipase-catalyzed synthesis of -sitostanol esters by response surface methodology. Food Chemistry 261: 139-148, 2018

Optimization of lipase-catalyzed synthesis of sorbitan acrylate using response surface methodology. Applied Biochemistry and Biotechnology 137-140(1-12): 595-609, 2008

Optimization of lipase-catalyzed synthesis of fructose stearate using response surface methodology. Artificial Cells, Nanomedicine, and Biotechnology 41(5): 344-351, 2014