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Targeting cancer cells with transferrin conjugates containing the non-toxic type 2 ribosome-inactivating proteins nigrin b or ebulin 1



Targeting cancer cells with transferrin conjugates containing the non-toxic type 2 ribosome-inactivating proteins nigrin b or ebulin 1



Cancer Letters 184(1): 29-35



Nigrin b and ebulin 1 are type 2 ribosome-inactivating proteins (RIPs) with 104 times less cellular and in vivo toxicity than ricin that are currently being considered for the construction of anti-cancer conjugates. Here we provide evidence that both RIPs can be used for the construction of conjugates directed to a target such as the transferrin receptor (TfR), which is over-expressed in cancer cells. Nigrin b- and ebulin 1-transferrin conjugates were constructed with no substantial reduction in the translational inhibitory molecular activity of either RIPs. Conjugation with transferrin decreased the IC50 of the proteins from 3 X 10-7 M (nigrin b) and 1.5 X 10-8 M (ebulin 1) to 3.5 X 10-10 M in HeLa cells. Thus, both conjugates could be considered as useful tools for targeting TfR-over-expressing cancer cells.

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Accession: 011453141

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PMID: 12104045


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