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The C-terminal domain of the alpha subunit of Escherichia coli RNA polymerase is required for efficient Rho-dependent transcription terminationn

Kainz, M.; Gourse, R.L.

Journal of Molecular Biology 284(5): 1379-1390

1998

ISSN/ISBN: 0022-2836
Accession: 011465699
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We screened a collection of single alanine residue substitution mutants spanning the entire C-terminal domain of the alpha subunit (alphaCTD) of Escherichia coli RNA polymerase (RNAP) for defects in rho-dependent transcription termination at lambdatR1 in vivo and in vitro, and thereby identified a patch of amino acid residues in the aCTD required for efficient rho-dependent termination. NusA addition led to the stimulation of rho-dependent termination under our conditions in vitro. The termination defects of a few mutant RNAPs could be attributed to altered interactions with the NusA protein, but rho-dependent termination by most of the defective RNAPs was still stimulated normally by NusA. The NusA-enhanced transcription pausing behaviors of the mutant RNAPs did not always correlate with their rho-dependent termination phenotypes. We conclude that the alphaCTD is a target for interactions with NusA that influence both termination and pausing, but in addition it participates in rho-dependent transcription termination in a NusA-independent manner.

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Related References

Kainz, M.; Gourse, R.L. 1999: The C-terminal domain of the alpha subunit of Escherichia coli RNA polymerase is required for efficient rho-dependent transcription termination Journal of Molecular Biology 284(5): 1379-1390
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