+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

The alternatively spliced deltae13 transcript of the rabbit calcitonin receptor dimerizes with the C1a isoform and inhibits its surface expression

The alternatively spliced deltae13 transcript of the rabbit calcitonin receptor dimerizes with the C1a isoform and inhibits its surface expression

Journal of Biological Chemistry 278(25): 23085-23093

ISSN/ISBN: 0021-9258

PMID: 12686555

DOI: 10.1074/jbc.m211280200

Numerous alternatively spliced transcripts are generated from the gene for the G protein-coupled calcitonin receptor, and some of the splice variants show differences in receptor-mediated signaling events. This study showed that the DELTAe13 splice variant of the rabbit calcitonin receptor is expressed together with the more common C1a in osteoclast-like cells. Since other G protein-coupled receptors form homo- or heterodimers, we examined whether heterodimerization of the calcitonin receptor splice variants occurs and, if so, whether it affects the function of the receptor. Homodimers of both isoforms and DELTAe13/C1a heterodimers were detected by co-immunoprecipitation and fluorescence resonance energy transfer analysis. In contrast to the C1a isoform, the DELTAe13 isoform was not efficiently transported to the cell surface. When co-expressed with the C1a splice variant, the DELTAe13 isoform colocalized with the C1a isoform within the cell but not at the cell surface. Furthermore, the overexpression of the DELTAe13 variant led to a significant reduction of the C1a surface expression and consequently a reduction of the cAMP response and Erk phosphorylation after ligand stimulation. We therefore suggest that the DELTAe13 variant of the rabbit calcitonin receptor acts to regulate the surface expression of the C1a isoform.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 011475897

Download citation: RISBibTeXText

Related references

The Alternatively Spliced e13 Transcript of the Rabbit Calcitonin Receptor Dimerizes with the C1a Isoform and Inhibits Its Surface Expression. The Journal of Biological Chemistry 278(25): 085-93, 2003

The alternatively spliced CD64 transcript FcgammaRIb2 does not specify a surface-expressed isoform. European Journal of Immunology 29(1): 143-149, 1999

Identification and expression analysis of alternatively spliced new transcript isoform of Bax gene in mouse. Gene 621: 21-31, 2017

PGC-1alpha regulates the isoform mRNA ratio of the alternatively spliced thyroid hormone receptor alpha transcript. Journal of Molecular Endocrinology 37(2): 251-257, 2006

Isolation and expression of a novel alternatively spliced mu opioid receptor isoform, MOR-1F. FEBS Letters 466(2-3): 337-340, 2000

Preferential expression of alternatively spliced transcript of type II procollagen in the rabbit notochordal remnant and developing fibrocartilages. Biochimica et Biophysica Acta 1350(3): 253-258, 1997

A previously unidentified alternatively spliced isoform of t(8;21) transcript promotes leukemogenesis. Nature Medicine 12(8): 945-949, 2006

LAR tyrosine phosphatase receptor Expression of a transcript with an alternatively spliced exon and with tandem CAG repeats. Neurology 43(4 Suppl 2): A301, 1993

Differential expression of the alternatively spliced OPRM1 isoform μ-opioid receptor-1K in HIV-infected individuals. Aids 28(1): 19-30, 2014

Tissue-specific and ubiquitous promoters direct the expression of alternatively spliced transcripts from the calcitonin receptor gene. Journal of Biological Chemistry 276(25): 22663-22674, 2001

NCAM-180, the large isoform of the neural cell adhesion molecule of the mouse, is encoded by an alternatively spliced transcript. Nucleic Acids Research 16(10): 4217-4225, 1988

Human canonical CD157/Bst1 is an alternatively spliced isoform masking a previously unidentified primate-specific exon included in a novel transcript. Scientific Reports 7(1): 15923, 2017

Alternatively spliced MEFV transcript lacking exon 2 and its protein isoform pyrin-2d implies an epigenetic regulation of the gene in inflammatory cell culture models. Genetics and Molecular Biology 40(3): 688-697, 2017

Neural expression of a novel alternatively spliced and polyadenylated Gsα transcript. The Journal of Biological Chemistry 268(13): 9879-9885, 1993

Expression of a novel alternatively spliced UCP-2 transcript in osteogenic sarcoma. Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association 11(1): 51-57, 2006