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The choice of reference cell in the analysis of kinetic data using BIAcore

The choice of reference cell in the analysis of kinetic data using BIAcore

Analytical Biochemistry 271(1): 70-80

An important step in the analysis of sensorgram data for BIAcore experiments is the subtraction of reference cell data to remove the effects of the bulk shift on the sensorgram of interest. It is shown that this step can introduce errors in the measured kinetic constants. This phenomenon is investigated both theoretically and with experimental data.

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Accession: 011482725

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PMID: 10361006

DOI: 10.1006/abio.1999.4129

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