+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The effects of endothelin-1 on the cardiorespiratory physiology of the freshwater trout (Oncorhynchus mykiss) and the marine dogfish (Squalus acanthias)



The effects of endothelin-1 on the cardiorespiratory physiology of the freshwater trout (Oncorhynchus mykiss) and the marine dogfish (Squalus acanthias)



Journal of Comparative Physiology B Biochemical Systemic and Environmental Physiology 171(8): 623-634



The aim of the present study was to evaluate the effects of endothelin-1-elicited cardiovascular events on respiratory gas transfer in the freshwater rainbow trout (Oncorhynchus mykiss) and the marine dogfish (Squalus acanthias). In both species, endothelin-1 (666 pmol kg-1) caused a rapid (within 4 min) reduction (ca. 30-50 mmHg) in arterial blood partial pressure of O2. The effects of endothelin-1 on arterial blood partial pressure of CO2 were not synchronised with the changes in O2 partial pressure and the responses were markedly different in trout and dogfish. In trout, arterial CO2 partial pressure was increased transiently by apprx1.0 mmHg but the onset of the response was delayed and occurred 12 min after endothelin-1 injection. In contrast, CO2 partial pressure remained more-or-less constant in dogfish after injection of endothelin-1 and was increased only slightly (apprx0.1 mmHg) after 60 min. Pre-treatment of trout with bovine carbonic anhydrase (5 mg ml-1) eliminated the increase in CO2 partial pressure that was normally observed after endothelin-1 injection. In both species, endothelin-1 injection caused a decrease in arterial blood pH that mirrored the changes in CO2 partial pressure. Endothelin-1 injection was associated with transient (trout) or persistent (dogfish) hyperventilation as indicated by pronounced increases in breathing frequency and amplitude. In trout, arterial blood pressure remained constant or was decreased slightly and was accompanied by a transient increase in systemic resistance, and a temporary reduction in cardiac output. The decrease in cardiac output was caused solely by a reduction in cardiac frequency; cardiac stroke volume was unaffected. In dogfish, arterial blood pressure was lowered by apprx10 mmHg at 6-10 min after endothelin-1 injection but then was rapidly restored to pre-injection levels. The decrease in arterial blood pressure reflected an increase in branchial vascular resistance (as determined using in situ perfused gill preparations) that was accompanied by simultaneous decreases in systemic resistance and cardiac output. Cardiac frequency and stroke volume were reduced by endothelin-1 injection and thus both variables contributed to the changes in cardiac output. We conclude that the net consequences of endothelin-1 on arterial blood gases result from the opposing effects of reduced gill functional surface area (caused by vasoconstriction) and an increase in blood residence time within the gill (caused by decreased cardiac output.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011500469

Download citation: RISBibTeXText

DOI: 10.1007/s003600100213


Related references

The effects of endothelin-1 on the cardiorespiratory physiology of the freshwater trout (Oncorhynchus mykiss) and the marine dogfish (Squalus acanthias). Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology 171(8): 623-634, 2002

The effects of endothelin-1 on the cardiorespiratory physiology of the freshwater trout and the marine dogfish. Journal of Comparative Physiology B Biochemical Systemic & Environmental Physiology 171(8): 623-634, 2001

Does gill boundary layer carbonic anhydrase contribute to carbon dioxide excretion: a comparison between dogfish (Squalus acanthias) and rainbow trout (Oncorhynchus mykiss). Journal of Experimental Biology 202(Pt 6): 749-756, 1999

The physiology of waterborne silver toxicity in freshwater rainbow trout (Oncorhynchus mykiss): 1. The effects of ionic Ag+. Aquatic Toxicology 35(2): 93-109, 1996

Branchial CO(2) receptors and cardiorespiratory adjustments during hypercarbia in Pacific spiny dogfish (Squalus acanthias). Journal of Experimental Biology 204(Pt 8): 1519-1527, 2001

Spiny dogfish Squalus acanthias biological characterization and exploitation state Caracterizacion biologica y estado de explotacion del tiburon espinoso Squalus acanthias. Unknown, 2004

Characterization of an endothelin ET(B) receptor in the gill of the dogfish shark Squalus acanthias. Journal of Experimental Biology 202(Pt 24): 3605-3610, 1999

The physiology of waterborne silver toxicity in freshwater rainbow trout (Oncorhynchus mykiss): 2. The effects of silver thiosulfate. Aquatic Toxicology 35(2): 111-125, 1996

Long-term hypoxia exposure alters the cardiorespiratory physiology of steelhead trout (Oncorhynchus mykiss), but does not affect their upper thermal tolerance. Journal of Thermal Biology 68(Pt B): 149-161, 2017

Comparison of metabolic fate of exogenous ammonia in an elasmobranch (Squalus acanthias) and a teleost (Oncorhynchus mykiss). 2007

The capacity of the kidney of the marine dogfish, Squalus acanthias, to secrete hydrogen ion. Journal of Cellular and Comparative Physiology 53: 205-213, 1959

Effect of Diets Containing Dogfish ( Squalus acanthias ) Meal on the Mercury Content and Growth of Pen-Reared Coho Salmon ( Oncorhynchus kisutch ). Journal of the Fisheries Research Board of Canada 33(8): 1771-1778, 1976

Effect of diets containing dogfish squalus acanthias meal on the mercury content and growth of pen reared coho salmon oncorhynchus kisutch. Journal of the Fisheries Research Board of Canada 33(8): 1771-1778, 1976

Functional anatomy and physiology of the musculature of the trout Oncorhynchus mykiss Funktionelle Anatomie und Physiologie der Muskulatur der Forelle Oncorhynchus mykiss. Biona Report, 11: 285-310, 1997

Effects of lactate ions on the cardiorespiratory system in rainbow trout ( Oncorhynchus mykiss ). American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 316(5): R607-R620, 2019