EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

The homeodomain protein Barx2 contains activator and repressor domains and interacts with members of the CREB family


The homeodomain protein Barx2 contains activator and repressor domains and interacts with members of the CREB family



Journal of Biological Chemistry 275(28): 21737-21745



ISSN/ISBN: 0021-9258

PMID: 10781615

DOI: 10.1074/jbc.m909998199

Barx1 and Barx2 are homeodomain proteins originally identified using regulatory elements of genes encoding certain cell adhesion molecules (CAMs). In the present study, we characterize regions of Barx2 that bind to regulatory elements of genes encoding three CAMs, L1, neuron-glia CAM (Ng-CAM), and neural CAM (N-CAM), and identify domains of Barx2 that regulate N-CAM transcription. The homeodomain of Barx2 was sufficient for binding to homeodomain binding sites (HBS) from all three CAM genes. The presence of a 17-amino acid Barx basic region resulted in a 2-fold decrease in binding to HBS sequences from the Ng-CAM and L1 genes, whereas it led to a 6.5-fold increase in binding to the HBS from the N-CAM promoter. Thus, the Barx basic region influences the strength and specificity of Barx2 binding to DNA. In co-transfection experiments, Barx2 repressed N-CAM promoter activity. A 24-residue N-terminal region of Barx2 was essential for repression. When this region was absent, Barx2 activated the N-CAM promoter. A 63-residue C-terminal domain was required for this activation. In GST pull-down experiments, Barx2 bound to proteins of the CREB family, CREB1 and ATF2. Overall, these findings provide a framework for understanding developmental and physiological contexts that influence repressor or activator functions of Barx2.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011512428

Download citation: RISBibTeXText

Related references

Characterization of human activating transcription factor 4, a transcriptional activator that interacts with multiple domains of cAMP-responsive element-binding protein (CREB)-binding protein. Journal of Biological Chemistry 272(38): 24088-24095, 1997

The homeodomain transcription factor NK-4 acts as either a transcriptional activator or repressor and interacts with the p300 coactivator and the Groucho corepressor. Journal of Biological Chemistry 274(44): 31543-31552, 1999

CREB-binding [corrected] protein interacts with the homeodomain protein Cdx2 and enhances transcriptional activity. Journal of Biological Chemistry 274(11): 7196-7199, 1999

I-mf, a novel myogenic repressor, interacts with members of the MyoD family. Cell 86(5): 731-741, 1996

Contextual interactions determine whether the Drosophila homeodomain protein, Vnd, acts as a repressor or activator. Nucleic Acids Research 33(1): 1-12, 2005

The proto-oncoprotein SYT interacts with SYT-interacting protein/co-activator activator (SIP/CoAA), a human nuclear receptor co-activator with similarity to EWS and TLS/FUS family of proteins. Journal of Biological Chemistry 280(52): 42863-42876, 2005

Human ZFM1 protein is a transcriptional repressor that interacts with transcription activation domain of stage-specific activator protein. The Journal of Biological Chemistry 273(12): 68-77, 1998

Human ZFM1 protein is a transcriptional repressor that interacts with the transcription activation domain of stage-specific activator protein. Journal of Biological Chemistry 273(12): 6868-6877, 1998

Multiple domains of repressor activator protein 1 contribute to facilitated binding of glycolysis regulatory protein 1. Proceedings of the National Academy of Sciences of the United States of America 95(24): 14112-14117, 1998

Identification of a novel SNF2/SWI2 protein family member, SRCAP, which interacts with CREB-binding protein. Journal of Biological Chemistry 274(23): 16370-16376, 1999

Mutations that define the optimal half-site for binding yeast GCN4 activator protein and identify an ATF/CREB-like repressor that recognizes similar DNA sites. Molecular and Cellular Biology 10(10): 5077-5086, 1990

Combination analysis of activator protein-1 family members, Sp1 and an activator protein-2alpha-related factor binding to different regions of the urokinase receptor gene in resected colorectal cancers. Clinical Cancer Research: An Official Journal of the American Association for Cancer Research 11(24 Pt 1): 8538-8548, 2005

The hepatitis B virus X protein functionally interacts with CREB-binding protein/p300 in the regulation of CREB-mediated transcription. Journal of Biological Chemistry 282(7): 4277-4287, 2007

The t(5;17) acute promyelocytic leukemia fusion protein NPM-RAR interacts with co-repressor and co-activator proteins and exhibits both positive and negative transcriptional properties. Blood 95(8): 2683-2690, 2000

The proline-rich homeodomain protein recruits members of the Groucho/Transducin-like enhancer of split protein family to co-repress transcription in hematopoietic cells. Journal of Biological Chemistry 279(33): 34938-34947, 2004