+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The role of apoptosis within the retina of coronavirus-infected mice



The role of apoptosis within the retina of coronavirus-infected mice



Investigative Ophthalmology & Visual Science 41(10): 3011-3018



Purpose. To evaluate the possible roles of apoptosis in the murine retinopathy induced by coronavirus. Methods. Mice were inoculated with virus intravitreally. Mouse eyes harvested at varying times after inoculation were evaluated for apoptotic and immunologic events by hematoxylin and eosin staining, immunohistochemical staining, in situ terminal deoxynucleotidyltransferase dUTP nickend labeling (TUNEL) assay, and electron microscopy. Isolated retinas were analyzed for infectious virus and for expression of apoptosis-associated genes. Results. The number of apoptotic events was significantly elevated in infected eyes from BALB/c and CD-1 mouse strains, reaching a maximum at days 6 through 10, and returning to normal levels at day 20. The majority of apoptotic cells were observed in the outer nuclear layer of the infected retina. In contrast, few apoptotic cells were observed in normal or mock-injected mouse eyes. Apoptotic events within the retina were associated with the presence of viral antigen, infiltration of CD8+ T cells, and clearance of infectious virus. Reverse transcription-polymerase chain reaction (RT-PCR) analysis identified the upregulation of Fas ligand (FasL) and granzyme B mRNAs within the infected retinas. The development of apoptosis, regulative gene expression, and viral clearance were similar in both retinal degeneration-susceptible (BALB/c) and -resistant (CD-1) mice. Conclusions. Retinal apoptosis was associated with retinal inflammation, a decrease in infectious virus, and upregulation of genes associated with CTL killing. These studies indicate that retinal apoptosis may be one of the host mechanisms that contribute to limiting this retinal infection.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 011543453

Download citation: RISBibTeXText

PMID: 10967058


Related references

Role of IFN-gamma responsiveness in CD8 T-cell-mediated viral clearance and demyelination in coronavirus-infected mice. Journal of Neuroimmunology 194(1-2): 18-26, 2008

Role of IFN-gamma responsiveness in CD8 T cell-mediated viral clearance and demyelination in coronavirus-infected mice. Journal of Neuroimmunology 194(1-2): 18-26, 2008

Induction of apoptosis in murine coronavirus-infected cultured cells and demonstration of E protein as an apoptosis inducer. Journal of Virology 73(9): 7853-7859, 1999

Role of endothelin-1 in glial cell proliferation and apoptosis in retina of db/db mice. Diabetes 52(Suppl. 1): A201, 2003

Selection of CTL escape mutants in mice infected with a neurotropic coronavirus: quantitative estimate of TCR diversity in the infected central nervous system. Journal of Immunology 163(11): 6106-6113, 1999

Induction of apoptosis in murine coronavirus-infected 17Cl-1 cells. Advances in Experimental Medicine and Biology 494: 615-620, 2001

In vitro detection of apoptosis in monocytes/macrophages infected with human coronavirus. Clinical and Diagnostic Laboratory Immunology 9(6): 1392-1395, 2002

Retina radioresistance: whole-body exposure of mice to gamma-irradiation induces the retina DNA damage, accumulation of p53 protein followed by DNA repair rather than the apoptosis. Radiatsionnaia Biologiia Radioecologiia 48(6): 698-704, 2009

Role of oxidative stress and apoptosis in the placental pathology of Plasmodium berghei infected mice. Plos one 7(3): E32694, 2012

Immune studies with coronavirus sd infected mice. Journal of Neurology 232(Suppl.): 49, 1985

The role of overproduction of nitric oxide in apoptosis of BALB/c mice macrophages infected with Leishmania major in vitro. Iranian Journal of Allergy, Asthma and Immunology 2(4): 209-214, 2003

The role of overproduction of nitric oxide in apoptosis of BALB/c mice macrophages infected with leishmania major in vitro. Iranian Journal of Allergy Asthma and Immunology 2(4): 209-214, 2003

Vacuolar degeneration in mice infected with a coronavirus JHM-CC strain. Neurology 40(6): 904-910, 1990

Structural and biological basis of CTL escape in coronavirus-infected mice. Journal of Immunology 180(6): 3926-3937, 2008