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Time-resolved site-directed spin-labeling studies of bacteriorhodopsin: Loop-specific conformational changes in M

Time-resolved site-directed spin-labeling studies of bacteriorhodopsin: Loop-specific conformational changes in M

Biochemistry 39(5): 1120-1127

A spin-label at site 101 in the C-D loop of bacteriorhodopsin was previously found to detect a conformational change during the M fwdarw N transition (Steinhoff, H. -J., Mollaaghababa, R., Altenbach, C., Hideg, K., Krebs, M. P., Khorana, H. G., and Hubbell, W. L. (1994) Science 266, 105-107). We have extended these time-resolved electron paramagnetic resonance studies in purple membranes by analyzing conformational changes detected by a spin-label at another site in the C-D loop (103), and at sites in the A-B loop (35), the D-E loop (130), and the E-F loop (160). In addition, we have investigated the motion detected by a spin-label at site 101 in a D96A mutant background that has a prolonged M intermediate. We find that among the examined sites, only spin-labels in the C-D loop detect a significant change in the local environment after the rise of M. Although the D96A mutation dramatically prolongs the lifetime of the M intermediate, it does not perturb either the structure of bacteriorhodopsin or the nature of the light-activated conformational change detected by a spin-label at site 101. In this mutant, a conformational change is detected during the lifetime of M, when no change in the 410 nm absorbance is observed. These results provide direct structural evidence for the heterogeneity of the M population in real time, and demonstrate that the motion detected at site 101 occurs in M, prior to Schiff base reprotonation.

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Accession: 011571359

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PMID: 10653658

DOI: 10.1021/bi991963h

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