+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Vasopressin-deficient suprachiasmatic nucleus grafts reinstate circadian rhythmicity in suprachiasmatic nucleus-lesioned arrhythmic rats

Vasopressin-deficient suprachiasmatic nucleus grafts reinstate circadian rhythmicity in suprachiasmatic nucleus-lesioned arrhythmic rats

Neuroscience 89(2): 375-385

It was investigated whether grafts of the suprachiasmatic nucleus could re-instate circadian rhythmicity in the absence of its endogenous vasopressin production and whether the restored rhythm would have the long period length of the donor. Grafts of 17-days-old vasopressin-deficient homozygous Brattleboro rat fetuses, homotopically placed in arrhythmic suprachiasmatic nucleus-lesioned Wistar rats, re-instated circadian drinking rhythm within 20-50 days similar as seen for grafts of heterozygous control fetuses. Period length of the recovered rhythm revealed a similar difference (average 24.3 vs 23.8 h) as reported for the rhythm between the adult Brattleboro genotypes. In all transplants, also those of the two-third non-recovery rats, a surviving suprachiasmatic nucleus was visible as a vasoactive intestinal polypeptide-positive neuronal cell cluster, whereas heterozygous transplants also revealed the complementary vasopressinergic cell part. Explanation of the absence of recovery failed since no undisputable correlation emerged between recovery of rhythm and vasoactive intestinal polypeptide, vasopressin and/or somatostatin immunocytochemical characteristics of the suprachiasmatic nucleus of the transplant. Special focus on the somatostatinergic neurons revealed their presence only occasionally near or in between the vasoactive intestinal polypeptidergic and (in the case of heterozygous grafts) vasopressinergic cell cluster. However their aberrant cytoarchitectural position appeared not to have affected the possibility to restore drinking rhythm of the suprachiasmatic nucleus-lesioned arrhythmic rat. It was concluded that grafted Brattleboro fetal suprachiasmatic nucleus develop their intrinsic rhythm conform their genotype and that vasopressin is not a crucial component in the maintenance nor in the transfer of circadian activity of the biological clock for drinking activity. Vasopressin of the suprachiasmatic nucleus may instead serve modulation within the circadian system.

(PDF emailed within 0-6 h: $19.90)

Accession: 011637077

Download citation: RISBibTeXText

PMID: 10077320

DOI: 10.1016/s0306-4522(98)00300-5

Related references

Circadian rhythmicity of vasopressin levels in the cerebrospinal fluid of suprachiasmatic nucleus-lesioned and -grafted rats. Journal of Biological Rhythms 14(1): 28-36, 1999

Functional development of fetal suprachiasmatic nucleus grafts in suprachiasmatic nucleus-lesioned rats. Brain Research Bulletin. 31(1-2): 145-160, 1993

Location of the suprachiasmatic nucleus grafts in rats which restored circadian rhythmicity after transplantation. Neuroscience Letters 118(1): 45-48, 1990

Light/dark-induced effects on behavioral rhythms in suprachiasmatic nucleus-lesioned rats irrespective of the presence of functional suprachiasmatic nucleus brain implants. Journal of Interdisciplinary Cycle Research 24(2): 118-136, 1993

Age of donor influences ability of suprachiasmatic nucleus grafts to restore circadian rhythmicity. Developmental Brain Research. 71(1): 45-52, 1993

Alterations in circadian rhythmicity of the vasopressin-producing neurons of the human suprachiasmatic nucleus (SCN) with aging. Brain Research 651(1-2): 134-142, 1994

Suprachiasmatic nucleus grafts restore circadian behavioral rhythms of genetically arrhythmic mice. Current Biology 13(8): 664-668, April 15, 2003

Vasopressin immunoreactivity, but not vasoactive intestinal polypeptide, correlates with expression of circadian rhythmicity in the suprachiasmatic nucleus of voles. Neuropeptides 41(4): 207-216, 2007

Not only vasopressin, but also the intracellular messenger protein kinase Calpha in the suprachiasmatic nucleus correlates with expression of circadian rhythmicity in voles. Neuropeptides 37(1): 57-65, 2003

Survival of suprachiasmatic nucleus grafts in rats lesioned and transplanted neonatally. Society for Neuroscience Abstracts 25(1-2): 215, 1999

Suprachiasmatic nucleus scn lesions abolish and fetal scn grafts restore circadian gnawing rhythms in scn lesioned adult hamsters. Society for Neuroscience Abstracts 17(1-2): 666, 1991

Expression of circadian rhythmicity correlates with the number of arginine-vasopressin-immunoreactive cells in the suprachiasmatic nucleus of common voles, Microtus arvalis. Brain Research 639(1): 93-101, 1994

Circadian rhythm of neuronal activity in suprachiasmatic nucleus slices from the vasopressin-deficient Brattleboro rat. Neuroscience. 75(2): 635-641, 1996

Meals incrementally and instantaneously reset circadian activity patterns in suprachiasmatic nucleus lesioned rats. Society for Neuroscience Abstracts 21(1-3): 954, 1995

Restricted feeding restores rhythmicity in the pineal gland of arrhythmic suprachiasmatic-lesioned rats. European Journal of Neuroscience 28(12): 2451-2458, 2009